Current Laboratory Methods in Neuroscience Research

Section 1: Neurohistological techniques.- 1. Brain tissue preparation, sectioning and staining.- 2. Brain stereotactic injections.- 3. Tract tracing at light and electron microscopy.- 4. Stereological analysis of neurological tissues.- Section 2: In vitro preparations.- 5. Preparation and use of rodent hippocampal brain slices.- 6.  Single-Cell Neuronal Dissociation For Electrophysiological Studies.- 7. Isolation and Culture of Human Neurons, Microglia and Astrocytes.- 8. Isolation and Culture of Neural Stem/progenitor Cells.- 9. Isolation of mitochondria from brain tissue and cells.- 10. Isolation of synaptosomes from archived brain tissues.- 11. Xenopus oocytes isolation and microinjection.- Section 3: Leukocyte isolation and application in neuroscience.- 12. Centrifugal elutriation for studies of neuroimmunity.- 13. Flow cytometry in neuroscience research.- Section 4: Standard Laboratory Nucleic Acid and Protein Detections.- 14. Western Blotting Technique in Biomedical Research.- 15. Polymerase chain reaction (PCR) and Real-time RT PCR.- Section 5: Nanomedicine.- 16. Nanoformulations.- 17. Neuronanomedicine.- Section 6: Bioimaging.- 18. Confocal imaging of nerve cells.- 19. Magnetic resonance imaging (MRI).- 20. X-Ray, Positron Emission, and Single Photon Emission Tomographic Bioimaging.- 21. Non-Invasive Neurophysiological Imaging with Magnetoencephalography.- 22. Multiphoton Brain Imaging.- Section 7: Neuroelectrophysiology.- 23. Techniques for Extracellular Recordings.- 24. “Blind” patch-clamp recordings from rodent hippocampal slices.- 25. Whole-cell patch clamp recordings.- Section 8: Immunohistochemistry and autoradiography.- 26. Analysis of Receptor Binding And Quantitative Autoradiography.- 27. Techniques in Immunohistochemistry and immunocytochemistry.- Section 9: Analysis of Gene Expression.- 28. Protein identification by mass spectrometry: Proteomics.- 29. DNA Microarrays as a Tool for Neurosciences Research.- 30. Metabolomics .- 31.Bioinformatic Methods and Resources for Neuroscience Research.- Section 10: Animal Models: Behavior and Pathology.- 32. Motor function in rodent models of neurodegenerative disorders.- 33. Humanized mice.- 34. Animal models for PD and ALS.- 35. Animal models for Alzheimer’s Disease.- 36. Animal Models: Behavior and Pathology.