Plant Developmental Biology
Methods and Protocols
Paperback Engels 2016 9781493957347Samenvatting
Plants come in myriads of shapes and colors, and the beauty of plants has fascinated mankind for thousands of years. Long before Mendel discovered the laws of heritab- ity and Darwin developed his theory on evolution, the affection for ornamental plants led people to select alleles that establish novel plant forms. Today, plant developmental biology tries to discover the mechanisms that control the establishment of specialized cell types, tissues, and organs from the fertilized egg during a plant’s life. Although the underlying processes of cell proliferation and differentiation are similar in plants and a- mals, plants are different because their development is usually open, and its outcome is not the faithful repetition of a general plan but is strongly in?uenced by environm- tal conditions. In the last few decades, plant developmental biology has pinpointed a large number of developmental regulators and their interactions and the mechanisms that govern plant development start to emerge. In part, this progress was enabled by the advance of powerful molecular tools for a few model species, most importantly Arabidopsis. This volume of the Methods in Molecular Biology series provides a collection of protocols for many of the common experimental approaches in plant developmental bi- ogy. All chapters are written in the same format as that used in the Methods in Molecular TM Biology series. Each chapter opens with a description of the basic theory behind the method being described.
Specificaties
Lezersrecensies
Inhoudsopgave
Edited by Lars Hennig and Claudia Köhler
Contents
1. Growth protocols for model plants in developmental biology
Lars Hennig
2. Grafting as a research tool
Colin G. N. Turnbull
3. Viral-induced gene silencing as a reverse genetics tool to study gene function
Steven Bernacki, Mansour Karimi, Pierre Hilson and Niki Robertson
4. The CRE/lox system as a tool for developmental studies at the cell and tissue level
Guy Wachsman and Renze Heidstra
5. Inducible gene expression systems for plants
Lorenzo Borghi
6. Trichome development in Arabidopsis
Joachim F. Uhrig and Martin Hülskamp
7. Phenotyping the development of leaf area in Arabidopsis thaliana
Sarah J. Cookson, Olivier Turc, Catherine Massonnet and Christine Granier
8. Analyzing shoot apical meristem development
Cristel Carles, Chan Man Ha, Ji Hyung Jun, Elisa Fiume and Jennifer C. Fletcher
9. Analyzing floral meristem development
Elisa Fiume, Helena R. Pires, Jin Sun Kim and Jennifer C. Fletcher
10. Female gametophytic mutants: Diagnosis and characterization
Ronny Völz and Rita Groß-Hardt
11. Pollen tube development
Mark A. Johnson and Benedikt Kost
12. Analysis of root meristem size development
Serena Perilli and Sabrina Sabatini
13. Phenotypic characterization of photomorphogenic responses during plant development
Thomas Kretsch
14. Kinematic analysis of cell division and expansion
Bart Rymen, Frederik Coppens, Stijn Dhondt, Fabio Fiorani and Gerrit T.S. Beemster
15. Flowering time control
Yvonne Möller-Steinbach, Cristina Alexandre and Lars Hennig
16. mRNA detection by whole mount in situ hybridization WISH or sectioned tissue in situ hybridzation SISH in Arabidopsis
Yvonne Stahl and Rüdiger Simon
17. Immunolocalization of proteins in plants
Michael Sauer and Ji?í Friml
18. Detection of small non-coding RNAs
Tamas Dalmay
19. Quantitative real time PCR in plant developmental biology
Vivien Exner
20. Luciferase and green fluorescent protein reporter genes as tools todetermine protein abundance and intracellular dynamics
András Viczián and Stefan Kircher
21. Fluorescence-activated cell sorting in plant developmental biology
Anjali S. Iyer-Pascuzzi and Philip N. Benfey
22. Laser microdissection of paraffin embedded plant tissues for transcript profiling
Robert C. Day
23. Utilizing bimolecular fluorescence complementation BiFC to assay protein-protein interaction in plants
Nir Ohad and Shaul Yalovsky
24. The split luciferase complementation assay
Naohiro Kato and Jason Jones
25. Co-immunoprecipitation and protein blots
Erika Isono and Claus Schwechheimer
26. Probing protein-protein interactions with FRET-FLIM
Christoph Bücherl, José Aker, Sacco de Vries, Jan Willem Borst
27. Plant chromatin immunoprecipitation
Corina B. R. Villar and Claudia Köhler
28. Immunocytological analysis of chromatin in isolated nuclei
Penka Pavlova, Federico Tessadori, Hans J. de Jong and Paul Fransz
29. Bisulphite sequencing of plant genomic DNA
Ernst Aichinger and Claudia Köhler
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