Plant Transcription Factors
Methods and Protocols
Paperback Engels 2016 9781493958443Samenvatting
Recent years have seen significant advancements in the development of enabling technologies that facilitate the study of Transcription Factors (TFs). TFs are pivotal in the regulation of plant development, reproduction, intercellular signaling, response to environment, cell cycle, and metabolism. Plant Transcription Factors: Methods and Protocols offers a comprehensive approach by covering the basic concepts as well as the detailed protocols of a series of commonly used tools for investigating plant TFs. From discussing select TF families in plants to presenting approaches for identifying them, methods are covered to verify the function, to identify protein interactions in which TFs are involved, and how the interactions are mediated. Increasing examples of TFs that function non-cell-autonomously are being discovered and methods to assess intercellular trafficking are also addressed. A section is devoted to examining interaction with DNA, and the volume concludes with a discussion of directed evolution to generate transcription factors that can more efficiently control desired processes. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and accessible, Plant Transcription Factors: Methods and Protocols serves as an ideal guide to seasoned plant molecular biologists as well as scientists new to the field of TFs and provides many necessary methods to all scientists who are interested in exploring the functions of transcription factors.
Specificaties
Lezersrecensies
Inhoudsopgave
1. MADS & More: Transcription Factors that Shape the Plant
Rainer Melzer and Günter Theißen
Section II. Identification of Transcription Factors
2. In Silico Mining and PCR Based Approaches to Transcription Factor Discovery in Non-Model Plants: Gene Discovery of the WRKY Transcription Factors in Conifers
Jun-Jun Liu and Yu Xiang
3. Isolation of Plant Transcription Factors Using a Yeast One-Hybrid System
Tatiana Pyvovarenko and Sergiy Lopato
4. A Transposon Based Activation Tagging System for Gene Function Discovery in Arabidopsis
Nayelli Marsch-Martínez
Section III. Functional Verification
5. CRES-T, An Effective Gene Silencing System Utilizing Chimeric Repressors
Nobutaka Mitsuda, Kyoko Matsui, Miho Ikeda, Masaru Nakata, Yoshimi Ooshima, Yukari Nagatoshi, and Masaru Ohme-Takagi
6. Analysis of a Transcription Factor Using Transient Assay in Arabidopsis Protoplasts
Yuji Iwata, Mi-Hyun Lee, and Nozomu Koizumi
7. Microarray-Based Identification of Transcription Factor Target Genes
Maartje Gorte, Anneke Horstman, Robert B. Page, Renze Heidstra, Arnold Stromberg, and Kim Boutilier
Section IV. Protein-Protein Interaction and Other Means for Activity Control
8. Yeast Protein-Protein Interaction Assays and Screens
Stefan de Folter and Richard G.H. Immink
9. Mapping Functional Domains of transcription factors
Ling Zhu and Enamul Huq
10. Bimolecular fluorescence complementation as a tool to study interactions of regulatory proteins in plant protoplasts
Sitakanta Pattanaik, Joshua R. Werkman, and Ling Yuan
11. Isolation of transcription factor complexes from Arabidopsis cell suspension cultures by tandem affinity purification
Jelle Van Leene, Dominique Eeckhout, Geert Persiau, Eveline Van De Slijke, Jan Geerinck, Gert Van Isterdael, Erwin Witters, and Geert De Jaeger
12. Assaying Transcription Factor Stability
Jasmina Kurepa and Jan A. Smalle
13. How to assess the intercellular trafficking of transcription factors
Munawar Ahmad, Won Kyong Cho, Yeonggil Rim, Lijun Huang, and Jae-Yean Kim
Section V. Protein-DNA Interaction
14. SELEX (Systematic Evolution of Ligands by EXponential Enrichment), as a powerful tool for deciphering the protein-DNA interaction space
Chenglin Chai, Zidian Xie, and Erich Grotewold
15. Footprinting and Missing Nucleoside Analysis of Transcription Factor-DNA Complexes
Ivana L. Viola and Daniel H. Gonzalez
16. Chromatin immunoprecipitation to verify or to identify In Vivo protein-DNA interactions
Yumei Zheng and Sharyn E. Perry
17. Visualizing and characterizing In Vivo DNA-Binding Events and Direct Target Genes of Plant Transcription Factors
Jose M. Muiño, Gerco C. Angenent, and Kerstin Kaufmann
18. Mapping In Vivo Protein-DNA Interactions in Plants by DamID, a DNA Adenine Methylation-Based Method
Sophie Germann and Valérie Gaudin
Section VI. Engineering Metablolic Pathways using Transcription Factors
19. Directed Evolution through DNA Shuffling for the Improvement and Understanding of Genes and Promoters
Joshua R. Werkman, Sitakanta Pattanaik, and Ling Yuan
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