Enzyme Engineering

<p>1. A Tripartite Fusion System for the Selection of Protein Variants with Increased Stability in vivo</p><p>Linda Foit and James Bardwell </p><p> </p><p>2. Determining Enzyme Kinetics via Isothermal Titration Calorimetry</p><p>Neil A. Demarse, Marie C. Killian, Lee D. Hansen, and Colette F. Quinn</p><p> </p><p>3. GFP Reporter Screens for the Engineering of Amino Acid Degrading Enzymes from Libraries Expressed in Bacteria</p><p>Olga Paley, Giulia Agnello, Jason Cantor, Tae Hyun Yoo, George Georgiou, and Everett Stone </p><p> </p><p>4. Flow Cytometric Assays for Interrogating LAGLIDADG Homing Endonuclease DNA Binding and Cleavage Properties</p><p>Sarah K. Baxter, Abigail R. Lambert, Andrew M. Scharenberg, and Jordan Jarjour </p><p> </p><p>5. TAL Effector Nuclease (TALEN) Engineering</p><p>Ting Li and Bing Yang</p><p> </p><p>6. In vitro Evolution of Enzymes</p><p>Misha V. Golynskiy, John C. Haugner III, Aleardo Morelli, Dana Morrone, and Burckhard Seelig </p><p> </p><p>7. Residue-Specific Incorporation of Unnatural Amino Acids into Proteins In vitro and In vivo</p><p>Amrita Signh-Blom, Randall A. Hughes, and Andrew. D. Ellington</p><p> </p><p>8. Reconstructing Evolutionary Adaptive Paths for Protein Engineering</p><p>Megan F. Cole, Vanessa E. Cox, Kelsey L. Gratton, and Eric A. Gaucher </p><p> </p><p>9. Oligonucleotide Recombination Enabled Site-Specific Mutagenesis in Bacteria</p><p>Bryan M. Swingle </p><p> </p><p>10. FX Cloning: A Versatile High-Throughput Cloning System for Characterization of Enzyme Variants </p><p>Eric R. Geertsma </p><p> </p><p>11. Use of Sulfolobus solfataricus PCNA Subunit Proteins to Direct the Assembly of Multimeric Enzyme Complexes</p><p>Hidehiko Hirakawa and Teruyuki Nagamune</p><p> </p><p>12. Gene Synthesis by Assembly of Deoxyuridine Containing Oligonucleotides </p><p>Romualdas Vaisvila and Jurate Bitinaite </p><p> </p><p>13. Protein Engineering: Single or Multiple Site-Directed Mutagenesis</p><p>Pei-Chung Hsieh and Romualdas Vaisvila</p><p> </p><p>14. Gene Assembly and Combinatorial Libraries in S. cerevisiae via Reiterative Recombination</p><p>Nili Ostrov, Laura M. Wingler, and Virginia Cornish </p><p> </p><p>15. Promiscuity-Based Enzyme Selection for Rational Directed Evolution Experiments</p><p>Sandeep Chakraborty, Renu Minda, Lipika Salaye, Abhaya M. dandekar, Swapan K. Bhattacharjee, and Basuthkar J. Rao</p><p> </p><p>16. Rational Protein Sequence Diversification by Multi-Codon Scanning Mutagenesis</p><p>Jia Liu and T. Ashton Cropp</p><p> </p><p>17. Screening Libraries for Improved Solubility: Using E. coli Dihydrofolate Reductase as a Reporter</p><p>Jian-Wei Liu and David Ollis </p><p> </p><p>18. In Vitro Directed Evolution of Enzymes Expressed by E. coli in Micro-Titre Plates</p>        Bradley J. Stevenson, Sylvia H.C. Yip, and David L. Ollis