Semaphorin Signaling

Semaphorins and their Signaling Mechanisms.- Isolating Fc-Tagged SEMA4D Recombinant Protein from 293FT Cells.- Expression and Purification of Class 7 Semaphorin and its Plexinc1 Receptor Using Baculovirus-Mediated Mammalian Cell Gene Transduction.- Immunoaffinity Purification of the Glycosylated Extracellular Fragment of Mouse Plexin A2 Produced in a Mammalian Expression Systems.- Plate-Based Assay for Measuring Direct Semaphorin-Neuropilin Interactions.- Characterizing Plexin Gtpase Interactions Using Gel Filtration, Surface Plasmon Resonance Spectrometry and Isothermal Titration Calorimetry.- In Vitro Assay for the Rap Gtpase Activating Protein Activity of the Purified Cytoplasmic Domain of Plexin.- Characterizing F-Actin Disassembly Induced by the Semaphorin Signaling Component.- Characterizing Erbb-2-Mediated Tyrosine Phosphorylation and Activation of Plexins.- Characterizing PKA-Mediated Phosphorylation of Plexin Using Purified Proteins.- Using Heterologous Cos-7 Cells to Identify Semaphorin Signaling Components.- Analysis of Semaphorin-Induced Growth Cone Collapse and Axon Growth Inhibition.- Using Rotary Shadow Electron Microscopy to Characterize Semaphorin-Mediated Growth Cone Collapse.- An Electrical Impedance-Based Method for Quantitative Real-Time Analysis of Semaphorin-Elicited Endothelial Cell Collapse.- Regulation of Cortical Dendrite Morphology and Spine Organization by Secreted Semaphorins – A Primary Culture Approach.- Characterizing Semaphorin Signaling Using Isolated Neurons in Culture.- Performing Axon Orientation Assays With Secreted Semaphorins And Other Guidance Cues.- Assays to Examine Transmembrane Semaphorin Function In Vitro.- Micro-CALI to Study Localized Roles of the Semaphorin Signaling Component CRMP in Axon Growth.- Visualizing and Characterizing Semaphorin Endocytic Events Using Quantum Dot Conjugated Proteins.- Visualization of Clathrin-Mediated Endocytosis during Semaphorin-Guided Axonal Growth.- Tracking Differential Endocytosis and Trafficking of Semaphorin Receptor Complexes in Responding Nerve Growth Cones.- Antibody-Feeding Assay: A Method to Track the Internalization of Neuropilin-1 and Other Cell Surface Receptors.- Photolithography-Based Substrate Microfabrication for Patterning Semaphorin 3A to Study Neuronal Development.- Characterization of Semaphorin 6A-Mediated Effects on Angiogenesis through Regulation of VEGF Signaling.- Studying the Effects of Semaphorins on Oligodendrocyte Lineage Cells.- Characterizing Semaphorin-Mediated Immune Responses Using an Antigen-Presentation Assay.- Podocyte Shape Regulation by Semaphorin 3A and MICAL-1.- In Vivo and In Vitro Knockdown Approachesin the Avian Embryo as a Means to Study Semaphorin Signaling.- Assaying the Action of Secreted Semaphorins on Vascular Permeability.- Characterization of the Effects of Semaphorin 4D Signaling on Angiogenesis.- Characterizing Semaphorin-Mediated Effects on Sensory and Motor Axon Pathfinding and Connectivity during Embryonic Development.- Experimental Approaches for Studying Semaphorin Signals in Tumor Growth and Metastasis in Mouse Models.- Characterizing Semaphorin Signaling In Vivo Using C. Elegans.