Eukaryotic Transcriptional and Post-Transcriptional Gene Expression Regulation

Fluorescence Reporter-Based Genome-Wide RNA Interference Screening to Identify Alternative Splicing Regulators.-&nbsp;Tandem Affinity Purification Approach Coupled To Mass Spectrometry to Identify Post-translational Modifications of Histones Associated with Chromatin-binding Proteins.-&nbsp;Efficient Preparation of High-Complexity ChIP-Seq Profiles from Early Xenopus Embryos.-&nbsp;Systematic Discovery Of Chromatin-bound Protein Complexes from ChIP-seq Datasets.-&nbsp;Assay for Transposase-Accessible Chromatin with high throughput sequencing (ATAC-seq) Protocol for Zebrafish Embryos.-&nbsp;Establishment of time- and cell-specific RNAi in Caenorhabditis elegans.-&nbsp;Cell-penetrating Peptide-mediated Delivery of Cas9 Protein and Guide RNA for Genome Editing.-&nbsp;Epigenetic Analysis of Endocrine Cell Subtypes from Human Pancreatic Islets.-&nbsp;eIF3 Regulation of Protein Synthesis, Tumorigenesis, and Therapeutic Response.-&nbsp;High Resolution Gene Expression Profiling of RNA Synthesis, Processing, and Decay by Metabolic Labeling of Newly Transcribed RNA using 4-thiouridine.-&nbsp;Accurate Detection of Differential Expression and Splicing using Low-level Features.-&nbsp;Profiling Changes in Histone Post-translational Modifications by Top-Down Mass Spectrometry.-&nbsp;Determining if an mRNA is a Substrate of Nonsense-mediated mRNA Decay in Saccharomyces cerevisiae.-&nbsp;Optimizing In Vitro Pre-mRNA 3' Cleavage Efficiency: Reconstitution from Anion-Exchange Separated HeLa Cleavage Factors and from Adherent HeLa Cell Nuclear Extract.-&nbsp;&nbsp;Unbiased Interrogation of 3D Genome Topology using Chromosome Conformation Capture Coupled to High-throughput Sequencing (3C-Seq).-&nbsp;Using an Inducible CRISPR-Cas9-KRAB Effector System to Dissect Transcriptional Regulation in Human Embryonic Stem Cells.-&nbsp;In vitro assay to Study Histone Ubiquitination during Transcriptional Regulation.-&nbsp;Determination of Alternate Splicing Events using Transcriptome Arrays.-&nbsp;Large-scale RNA Interference Screening to Identify Transcriptional Regulators of a Tumor Suppressor.- GeneTranscriptional Analysis-based Integrative Genomics Approach to Identify Tumor Promoting Metabolic Genes.<p><br></p>

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