Hypertension

Large Scale Transcriptome Analysis.-&nbsp;Methods to Assess Genetic Risk Prediction.-&nbsp;Microarray Analysis of Hypertension.-&nbsp;Tissue Proteomics in Vascular Disease.-&nbsp;Urine Metabolomics in Hypertension Research.-&nbsp;Systems Biology Approach in Hypertension Research.-&nbsp;Measurement of Angiotensin Peptides: HPLC, RIA.-&nbsp;Measurement of Angiotensin Converting Enzyme 2 Activity in Biological Fluid (ACE2).-&nbsp;Determining the Enzymatic Activity of Angiotensin-Converting Enzyme 2 (ACE2) in Brain Tissue and Cerebrospinal Fluid Using a Quenched Fluorescent Substrate.-&nbsp;Measurement of Cardiac Angiotensin II by Immunoassays, HPLC-chip/Mass Spectrometry and Functional Assays.-&nbsp;Analysis of the Aldosterone Synthase (CYP11B2) and 11b-Hydroxylase (CYP11B1) Genes.-&nbsp;Dopaminergic Immunofluorescence Studies in Kidney Tissue.-&nbsp;Techniques for the Evaluation of the Genetic Expression, Intracellular Storage and Secretion of Polypeptide Hormones with SpecialReference to the Natriuretic Peptides (Nps).-&nbsp;Intracellular Free Calcium Measurement Using Confocal Imaging.-&nbsp;Measuring T-type Calcium Channel Currents in Isolated Vascular Smooth Muscle Cells.-&nbsp;In Vitro Analysis of Hypertensive Signal Transduction:&nbsp; Kinase Activation, Kinase Manipulation, and Physiologic Outputs.-&nbsp;In vitro and in vivo Approaches to Assess Rho-kinase Activity.-&nbsp;NADPH Oxidases and Measurement of Reactive Oxygen Species.-&nbsp;Measurement of Superoxide Production and NADPH Oxidase Activity by HPLC Analysis of Dihydroethidium Oxidation.-&nbsp;Assessment of Caveolae/Lipid Rafts in Isolated Cells.-&nbsp;Isolation and Characterization of Circulating Microparticles by Flow Cytometry.-&nbsp;Isolation of Mature Adipocytes from White Adipose Tissue and Gene Expression Studies by Real-Time Quantitative RT-PCR.-&nbsp;Isolation and Differentiation of Murine Macrophages.-&nbsp;Isolation and Differentiation of Human Macrophages.-&nbsp;Isolation of Immune Cells for Adoptive Transfer.-&nbsp;Isolation and Culture of Endothelial Cells from Large Vessels.-&nbsp;Isolation and Culture of Vascular Smooth Muscle Cells from Small and Large Vessels.-&nbsp;Evaluation of Endothelial Dysfunction in vivo.-&nbsp;Vascular Reactive of Isolated Aorta To Study the Angiotensin-(1-7) Actions.-&nbsp;Generation of a Mouse Model with Smooth Muscle Cell Specific Loss of the Expression of< PPARγ.-&nbsp;Renal Delivery of Anti-microRNA Oligonucleotides in Rats.-&nbsp;In Vivo Analysis of Hypertension: Induction of Hypertension, In Vivo Kinase Manipulation And Assessment Of Physiologic Outputs.&nbsp;<p><br></p>