Type 3 Secretion Systems

<p>Introduction to Type III Secretion Systems.-&nbsp;Site-Directed Mutagenesis and its Application in Studying the Interactions of T3S Components.-&nbsp;Blue Native Protein Electrophoresis to Study the T3S System using Yersinia pestis as a Model.- In Vivo Photo-Cross-Linking to Study T3S Interactions Demonstrated using the Yersinia pestis T3S System.-&nbsp;Isolation of Type III Secretion System Needle Complexes by Shearing.-&nbsp;Use of Transcriptional Control to Increase Expression and Secretion of Heterologous Proteins in T3S Systems.-&nbsp;Characterization of Type Three Secretion System Translocator Interactions with Phospholipid Membranes.-&nbsp;Analysis of Type III Secretion System Secreted Proteins.-&nbsp;Fractionation Techniques to Examine Effector Translocation.- Measurement of Effector Protein Translocation using Phosphorylatable Epitope Tags and Phospho-Specific Antibodies.-&nbsp;A TAL-Based Reporter Assay for Monitoring Type III-Dependent Protein Translocationin Xanthomonas.-&nbsp;Subcellular Localization of Pseudomonas syringae pv. tomato Effector Proteins in Plants.-&nbsp;A Method for Characterizing the Type III Secretion System’s Contribution to Pathogenesis: Homologous Recombination to Generate Yersinia pestis Type III Secretion System Mutants.-&nbsp;Detecting Immune Responses to Type III Secretion Systems.-&nbsp;Recombinant Expression and Purification of the Shigella Translocator IpaB.-&nbsp;Expression and Purification of N-Terminally His-Tagged Recombinant Type III Secretion Proteins.-&nbsp;Mouse Immunization with Purified Needle Proteins from Type III Secretion Systems and the Characterization of the Immune Response to these Proteins.- Identification of the Targets of Type III Secretion System Inhibitors.-&nbsp;Detection of Protein Interactions in T3S Systems using Yeast 2-Hybrid Analysis.</p>