Hepatitis B Virus

NTCP-Reconstituted In Vitro HBV Infection System.- Hepatitis B Virus Infection of HepaRG Cells, HepaRG-hNTCP Cells and Primary Human Hepatocytes.- Live Cell Imaging Confocal Microscopy Analysis of HBV Myr-PreS1 Peptide Binding and Uptake in NTCP-GFP Expressing HepG2 Cells.- Intracytoplasmic Transport of Hepatitis B Virus Capsids.- A Homokaryon Assay for Nucleocytoplasmic Shuttling Activity of HBV Core Protein.- Analyses of HBV cccDNA Quantification and Modification.- Detection of HBV cccDNA Methylation from Clinical Samples by Bisulfite Sequencing and Methylation Specific PCR.- A T7 Endonuclease I Assay to Detect TALEN-Mediated Mutation of HBV cccDNA.- Detection of Hepatocyte Clones Containing Integrated Hepatitis B Virus DNA using Inverse Nested PCR.- Highly Sensitive Detection of HBV RNA in Liver Tissue by In Situ Hybridization.- Immunofluorescent Staining for the Detection of the Hepatitis B Core Antigen in Frozen Liver Sections of Human Liver Chimeric Mice.- Measuring Changes in Cytosolic Calcium Levels in HBV- and HBx-Expressing Cultured Primary Hepatocytes.- In Vitro Assays for RNA Binding and Protein Priming of Hepatitis B Virus Polymerase.- In Vitro Enzymatic and Cell Culture-Based Assays for Measuring Activity of HBV RNaseH Inhibitors.- Detection of Hepatitis B Virus Particles Released from Cultured Cells by Particle Gel Assay.- Microtiter-Format Assays for HBV Antigen Quantitation in Non-Clinical Applications.- Deep Sequencing of the Hepatitis B Virus Genome: Analysis of Multiple Samples by Implementation of the Illumina Platform.- Generation of Replication Competent Hepatitis B Virus Genome from Blood Samples for Functional Characterization.- Hydrodynamic HBV Transfection Mouse Model.- An ELISPOT-Based Assay to Measure HBV-Specific CD8+ T Cell Responses in Immunocompetent Mice.- Advanced Method for Isolation of Mouse Hepatocytes, Liver Sinusoidal Endothelial Cells andKupffer Cells.- Partial Hepatectomy and Castration of HBV Transgenic Mice.- Studying HBV Infection and Therapy in Immune Deficient NOD-Rag1-/-IL2RgammaC-null (NRG) Fumarylacetoacetate Hydrolase (Fah) Knockout Mice Transplanted with Human Hepatocytes.- Measurement of Antiviral Effect and Innate Immune Response during Treatment of Primary Woodchuck Hepatocytes.