Synthetic Protein Switches

<p>Synthetic Protein Switches: Theoretical and Experimental Considerations.-&nbsp;Construction of Allosteric Protein Switches by Alternate Frame Folding and Intermolecular Fragment Exchange.-&nbsp;Construction of Protein Switches by Domain Insertion and Directed Evolution.-&nbsp;Catalytic Amyloid Fibrils that Bind Copper to Activate Oxygen.-&nbsp;Ancestral Protein Reconstruction and Circular Permutation for Improving the Stability and Dynamic Range of FRET Sensors.-&nbsp;Method for Developing Optical Sensors Using a Synthetic Dye-Fluorescent Protein FRET Pair and Computational Modelling and Assessment.-&nbsp;Rational Design and Applications of Semi-Synthetic Modular Biosensors: SNIFITs and LUCIDs.-&nbsp;Ultrasensitive Firefly Luminescent Intermediate-Based Protein-Protein Interaction Assay (FlimPIA) Based on the Functional Complementation of Mutant Firefly Luciferases.-&nbsp;Quantitative and Dynamic Imaging of ATM Kinase Activity.-&nbsp;Creation of Antigen-Dependent β-Lactamase Fusion Protein Tethered by Circularly Permuted Antibody Variable Domains.-&nbsp;Protein and Protease Sensing by Allosteric Derepression.-&nbsp;DNA-Specific Biosensors Based on Intramolecular β-Lactamase-Inhibitor Complex Formation.-&nbsp;Engineering and Characterizing Synthetic Protease Sensors and Switches.-&nbsp;Characterizing Dynamic Protein-Protein Interactions Using the Genetically Encoded Split Biosensor Assay Technique Split TEV.-&nbsp;Development of a Synthetic Switch to Control Protein Stability in Eukaryotic Cells with Light.-&nbsp;Light-Regulated Protein Kinases Based on the CRY2-CIB1 System.-&nbsp;Yeast-Based Screening System for the Selection of Functional Light-Driven K⁺ Channels.-&nbsp;Primer-Aided Truncation for the Creation of Hybrid Proteins.-&nbsp;Engineering Small Molecule Responsive Split-Protein Kinases.-&nbsp;Directed Evolution Methods to Rewire Signaling Networks.</p>