Bacterial Protein Secretion Systems

Identification of Protein Secretion Systems in Bacterial Genomes using MacSyFinder.-&nbsp;Protein Sorting Prediction&nbsp;.-&nbsp;Cell Fractionation.-&nbsp;Defining Lipoprotein Localisation by Fluorescence Microscopy.-&nbsp;Components Sub-cellular Localization: Identification of Lipoproteins using Globomycin and Radioactive Palmitate.-&nbsp;Defining Membrane Protein Localization by Isopycnic Density Gradients.-&nbsp;Components Sub-cellular Localisation: Cell Surface Exposure.-&nbsp;Probing Inner Membrane Protein Topology by Proteolysis.-&nbsp;Mapping of Membrane Protein Topology by Substituted Cysteine Accessibility Method (SCAM^TM).-&nbsp;Defining Membrane Protein Topology using pho-lac Reporter Fusions.-&nbsp;In vivo and in vitro Protein-peptidoglycan Interactions.-&nbsp;Measure of Peptidoglycan Hydrolase Activity.-&nbsp;Protein-protein Interaction: Bacterial Two-Hybrid.-&nbsp;Protein-protein Interactions: Yeast two-hybrid.-&nbsp;Protein-protein Interactions: Cytology Two-hybrid.-&nbsp;Fusion Reporter Approaches to Monitor Transmembrane Helix Interactions in Bacterial Membranes.-&nbsp;Protein-protein Interactions: Co-immunoprecipitation.-&nbsp;Protein-protein Interaction: Tandem Affinity Purification in Bacteria.-&nbsp;Site-directed and Time-resolved Photocrosslinking in Cells Metabolically Labeled With Radioisotopes.-&nbsp;Protein-protein Interactions: Pull-down Assays.-&nbsp;Protein-protein interactions: Surface Plasmon Resonance.-&nbsp;Assessing Energy-dependent Protein Conformational Changes in the TonB System.-&nbsp;Defining Assembly Pathways by Fluorescence Microscopy.-&nbsp;Large Complexes: Cloning Strategy, Production, and Purification.-&nbsp;Shearing and Enrichment of Extracellular Type IV Pili.-&nbsp;Blue Native PAGE Analysis of Bacterial Secretion Complexes.-&nbsp;In situ Imaging of Bacterial Secretion Systems by Electron Cryo-tomography.-&nbsp;Structural Analysis of Protein Complexes by Cryo Electron Microscopy.-&nbsp;Bacterial Filamentous Appendages Investigated by Solid-state NMR Spectroscopy.-&nbsp;Identification&nbsp;of Effectors:&nbsp; Precipitation of Supernatant Material.-&nbsp;Energy Requirements for Protein Secretion via the Flagellar type-III Secretion System.-&nbsp;Screening for Secretion of the Type VI Secretion Protein Hcp by Enzyme-Linked Immunosorbent Assay and Colony Blot.-&nbsp;Effector Translocation: Cya&nbsp;Reporter Assay.-&nbsp;Monitoring Effector Translocation with the TEM-1 Beta-lactamase Reporter System.-&nbsp;Effector Translocation Assay: Differential Solubilization.-&nbsp;Quantitative Determination of Anti-bacterial Activity during Bacterial Co-culture.&nbsp; <p><br></p>