RNA Detection

<p> The Secret Life of RNA: Lessons from Emerging Methodologies.-&nbsp;Quantification of 2'-O-Me Residues in RNA using Next-Generation Sequencing (Illumina RiboMethSeq Protocol).-&nbsp;Identifying the m⁶A Methylome by Affinity Purification and Sequencing.-&nbsp;PARIS: Psoralen Analysis of RNA Interactions and Structures with High Throughput and Resolution.-&nbsp;Axon-TRAP-RiboTag: Affinity-Purification of Translated mRNAs from Neuronal Axons in Mouse In Vivo.-&nbsp;LCM-Seq: A Method for Spatial Transcriptomic Profiling using Laser Capture Microdissection Coupled with PolyA-Based RNA Sequencing.-&nbsp;Spatial Transcriptomics: Constructing a Single-Cell Resolution Transcriptome-Wide Expression Atlas.-&nbsp;Single mRNA Molecule Detection in Drosophila.-&nbsp;Detection and Automated Analysis of Single Transcripts at Subcellular Resolution in Zebrafish Embryos.-&nbsp;Super-Resolution Single Molecule FISH at the Drosophila Neuromuscular Junction.-&nbsp;Detection of mRNA and Associated Molecules by ISH-IEM on Frozen Sections.-&nbsp;Hybridization Chain Reaction for Direct mRNA Detection without Nucleic Acid Purification.-&nbsp;In Situ Detection of MicroRNA Expression with RNAscope Probes.-&nbsp;Padlock Probes to Detect Single Nucleotide Polymorphisms.-&nbsp;Quantifying Gene Expression in Living Cells with Ratiometric Bimolecular Beacons.-&nbsp;Optimizing Molecular Beacons for Intracellular Analysis of RNA.-&nbsp;Live Imaging of Nuclear RNPs in Mammalian Complex Tissue with ECHO-liveFISH.-&nbsp;In Vivo Visualization and Function Probing of Transport mRNPs using Injected FIT Probes.-&nbsp;Visualizing RNA in Live Bacterial Cells using Fluorophore- and Quencher-Binding Aptamers.-&nbsp;Method for Imaging Live-Cell RNA using an RNA Aptamer and a Fluorescent Probe.-&nbsp;RNA Live Imaging in the Model Microorganism Ustilago maydis.-&nbsp;Real-Time Fluorescence Imaging of Single-Molecule Endogenous Non-Coding RNA in Living Cells.-&nbsp;Live Imaging of mRNA Synthesis in Drosophila.-&nbsp;Imaging Newly Transcribed RNA in Cells by Using a Clickable Azide-Modified UTP Analog.-&nbsp;Detection of the First Round of Translation: The TRICK Assay.-&nbsp;Imaging Translation Dynamics of Single mRNA Molecules in Live Cells.-&nbsp;Systematic Detection of Poly(A)⁺ RNA-Interacting Proteins and Their Differential Binding.-&nbsp;Isolation and Characterization of Endogenous RNPs from Brain Tissues.-&nbsp;Individual Nucleotide Resolution UV Cross-Linking and Immunoprecipitation (iCLIP) to Determine Protein-RNA Interactions.-&nbsp;RNA Tagging: Preparation of High-Throughput Sequencing Libraries.-&nbsp;RAP-MS: A Method to Identify Proteins that Interact Directly with a Specific RNA Molecule in Cells.</p>