Kinase Signaling Networks

Optogenetic Control of Ras/Erk Signaling Using the Phy–PIF System.- Dissecting Kinase Effector Signaling Using the RapRTAP Methodology.- Single-Cell Imaging of ERK Signaling Using Fluorescent Biosensors.- Quantification of Cell Signaling Networks Using Kinase Activity Chemosensors.- Expression of Recombinant Phosphoproteins for Signal Transduction Studies.- Allosteric Modulation of Src-Family Kinases with ATP-Competitive Inhibitors.- Characterization of Ligand Binding to Pseudokinases Using a Thermal Shift Assay.- Proteomic Profiling of Protein Kinase Inhibitor Targets by Mass Spectrometry.- Utilizing the Luminex Magnetic Bead-Based Suspension Arrays for Rapid Multiplexed Phosphoprotein Quantification.- High-Content Imaging and RNAi Screens for Investigating Kinase Network Plasticity.- Analysis of Drug Resistance Using Kinome-Wide Functional Screens.- Identification and Validation of Driver Kinases from Next Generation Sequencing Data.- Label-Free Phosphoproteomic Approach for Kinase Signaling Analysis.- Cell-Specific Labeling for Analyzing Bidirectional Signaling by Mass Spectrometry.- Characterization of the Phospho-Adhesome by Mass Spectrometry-Based Proteomics.- Analysis of Phosphotyrosine Signaling Networks in Lung Cancer Cell Lines.- Targeted Analysis of Phosphotyrosine Signaling by Multiple-Reaction-Monitoring Mass Spectrometry.- Phosphoproteomic Analysis of Isolated Mitochondria in Yeast.- A Methodology for Comprehensive Analysis of Toll-Like Receptor Signaling in Macrophages.- Absolute Phosphorylation Stoichiometry Analysis by Motif-Targeting Quantitative Mass Spectrometry.- Identification of Plant Kinase Substrates Based on Kinase Assay-Linked Phosphoproteomics.- Mass Spectrometry Analysis of Spatial Protein Networks by Colocalization Analysis (COLA).- Development of Selected Reaction Monitoring Methods to Systematically Quantify Kinase Abundance and Phosphorylation Stoichiometry in Human Samples.- Analysis of Signaling Networks at the Single Cell Level Using Mass Cytometry.- Magnetic Resonance Spectroscopy (MRS)-Based Methods for Examining Cancer Metabolism in Response to Oncogenic Kinases Drug Treatment.- Deconstructing the Metabolic Networks of Oncogenic Signaling Using Targeted Liquid-Chromatography Tandem Mass Spectrometry (LC-MS/MS).- Modeling of Receptor Tyrosine Kinase Signaling: Computational and Experimental Protocols.- An Interdisciplinary Approach for Designing Kinetic Models of the Ras/MAPK Signaling Pathway.- Databases and Computational Tools for Evolutionary Analysis of Protein Phosphorylation.- Informatics Approaches for Predicting, Understanding, and Testing Cancer Drug Combinations.- Target Inhibition Maps Based on Responses to Kinase Inhibitors.- Partial Least Squares Regression Models for the Analysis of Kinase Signaling.</p>