Handbook of ELISPOT

Challenges in Developing Protein Secretion Assays at a Single-Cell Level.- To Count of Not to Count: ELISPOT Evaluation Revisited.- Essential Controls for ELISpot Assay.- Automatic Search of Spots and Color Classification in ELISPOT Assay.- Four Color ImmunoSpot ® Assays for Identification of Effector T Cell Lineages.- Detection of Cross-Reactive B Cells Using the FluoroSpot Assay.- Multiplex ImmunoSpot® Assays for the Study of Functional B Cell Subpopulations.- Detecting All Immunoglobulin Classes and Subclasses in Multiplex 7 Color ImmunoSpot® Assays.- Multiplexing T- and B-Cell FLUOROSPOT Assays: Experimental Validation of the Multi-Color ImmunoSpot® Software Based on Center of Mass Distance Algorithm.- Multi-Color FLUOROSPOT Counting Using ImmunoSpot® Fluoro-X™ Suite.- B-Cell ELISpot Assay to Quantify Antigen-Specific Antibody-Secreting Cells in Human Peripheral Blood Mononuclear Cells.- Identification of Novel Mycobacterial Targets for Murine CD4+T Cells by IFN ELISPOT.- ELISPOT-Based “Multi-Color FluoroSpot” to Study Type-Specific and Cross-Reactive Responses in Memory B Cells after Dengue and Zika Virus Infections.- Cultured ELISpot Assay to Investigate Dengue Virus Specific T Cell Responses.- Ex Vivo ELISpot Assay to Investigate Dengue Virus Specific T Cell Responses.- Ex Vivo ELISpot Assay to Investigate iNKT Cell Responses in Acute Dengue Infection.- Dendritic Cell-Based ELISpot Assay for Assessing T Cell-IFN- γ Responses in Human Peripheral Blood Mononuclear Cells to Dengue Envelope Proteins.- Utilization of Feline ELISpot to Evaluate the Immunogenicity of a T Cell-Based FIV MAP Vaccines.- Detection and Quantification of Influenza A/H1N1 Virus-Specific Memory B Cells in Human PBMCs using ELISpot Assay.