ADP-ribosylation and NAD+ Utilizing Enzymes

Vitamin B3 in Health and Disease: Toward the Second Century of Discovery.- Monitoring Poly(ADP-Ribosyl)ation in Response to DNA Damage in Live Cells Using Fluorescently-Tagged Macrodomains.- In Vitro Techniques for ADP-Ribosylated Substrate Identification.- Assessment of Intracellular Auto-Modification Levels of ARTD10 using Mono-ADP-Ribose-Specific Macrodomains 2 and 3 of Murine Artd8.- Biochemical and Biophysical Assays of PAR-WWE Domain Interactions and Production of iso-ADPr for PAR Binding Analysis.- Assays for NAD-Dependent Reactions and NAD Metabolites.- Generating Protein-Linked and Protein-Free Mono-, Oligo-, and Poly(ADP-Ribose) In Vitro.- Methods to Study TCDD-Inducible Poly-ADP-Ribose Polymerase (TIPARP) Mono-ADP-Ribosyltransferase Activity.- Dictyostelium as a Model to Assess Site-Specific ADP-Ribosylation Events.- Mono-ADP-Ribosylation Catalyzed by Arginine-Specific ADP-Ribosyltransferases.- Monitoring Expression andEnzyme Activity of Ecto-ARTCs.- ADP-Ribosyl-Acceptor Hydrolase Activities Catalyzed by the ARH Family of Proteins.- Mono-ADP-Ribosylhydrolase Assays.- Hydrolysis of ADP-Ribosylation by Macrodomains.- HPLC-Based Enzyme Assays for Sirtuins.- Small Molecule Screening Assay for Mono-ADP-Ribosyltransferases.- A Simple, Sensitive, and Generalizable Plate Assay for Screening PARP Inhibitors.- Non-Localized Searching of HCD Data for Fast and Sensitive Identification of ADP-Ribosylated Peptides.- Quantitative Determination of MAR Hydrolase Residue Specificity In Vitro by Tandem Mass Spectrometry.- Detection of ADP-Ribosylating Bacterial Toxins.- Preparation of Recombinant Alphaviruses for Functional Studies of ADP-Ribosylation.- Monitoring the Sensitivity of T Cell Populations towards NAD+ Released during Cell Preparation.- Identifying Target RNAs of PARPs.- ADPr-Peptide Synthesis.- Identifying Genomic Sites of ADP-Ribosylation Mediated by Specific Nuclear PARP Enzymes Using Click-ChIP.- Methods for Using a Genetically-Encoded Fluorescent Biosensor to Monitor Nuclear NAD+.