Antibody Engineering

Antibody Design and Humanization via In Silico Modeling.- Antibody Affinity Maturation by Computational Design.- Use of IMGT® Databases and Tools for Antibody Engineering and Humanization.- Construction of Human Naïve Antibody Gene Libraries.- Construction of Synthetic Antibody Libraries.- Construction of Histidine-Enriched Shark IgNAR Variable Domain Antibody Libraries for the Isolation of pH-Sensitive vNAR Fragments.- Display Technologies for Generation of Ig Single Variable Domains.- A Streamlined Approach for the Construction of Large Yeast Surface Display Fab Antibody Libraries.- Phage Display and Selections on Purified Antigens.- Selection of Antibodies to Transiently Expressed Membrane Proteins Using Phage Display.- Selection of Antibody Fragments against Structured DNA by Phage Display.- Selection of Antibody Fragments by Yeast Display.- Rapid Selection of High Affinity Antibody scFv Fragments Using Ribosome Display.- In Vitro Selection of Single Domain Antibody (VHH) Using cDNA Display.- Sequencing and Affinity Determination of Antigen-Specific B Lymphocytes from Peripheral Blood.- Expression of IgG Monoclonals with Engineered Immune Effector Functions.- An IRES-Mediated Tricistronic Vector for Efficient Generation of Stable, High Level Monoclonal Antibody Producing CHO DG44 Cell Lines.- Production, Purification, and Characterization of Antibody-TNF Superfamily Ligand Fusion Proteins.- Chemoenzymatic Defucosylation of Therapeutic Antibodies for Enhanced Effector Functions Using Bacterial α-Fucosidases.- Fc Glyco- and Fc Protein-Engineering: Design of Antibody Variants with Improved ADCC and CDC Activity.- Fc Engineering: Tailored Synthetic Human IgG1-Fc Repertoire for High Affinity Interaction with FcRn at pH 6.0.- Measuring Antibody-Antigen Binding Kinetics Using Surface Plasmon Resonance.- Parallel Evolution of Antibody Affinity and Thermal Stability for Optimal Biotherapeutic Development.- The Use of Somatic Hypermutation for the Affinity Maturation of Therapeutic Antibodies.- Selection and Use of Intracellular Antibodies.- Site-Specific Radioactive Labeling of Nanobodies.</p>