Phytoplasmas

<p>Phytoplasmas: An Introduction.-&nbsp;Micro-tom Tomato Grafting for Stolbur-phytoplasma Transmission: Different Grafting Techniques.-&nbsp;Phytoplasma Transmission: Insect Rearing and Infection Protocols.-&nbsp;Sampling Methods for Leafhopper, Planthopper, and Psyllid Vectors.-&nbsp;Symptoms of Phytoplasma Diseases.-&nbsp;Comparison of Different Procedures for DNA Extraction for Routine Diagnosis of Phytoplasmas.-&nbsp;Standard Detection Protocol: PCR and RFLP Analyses Based on 16S rRNA Gene.-&nbsp;PCR-based Sequence Analysis on Multiple Genes other than 16S rRNA Gene for Differentiation of Phytoplasmas.-&nbsp;Real-time PCR Protocol for Phytoplasma Detection and Quantification.-&nbsp;Duplex TaqMan Real-Time PCR for Rapid Quantitative Analysis of a Phytoplasma in its Host Plant without External Standard Curves.-&nbsp;A&nbsp;Multiplex-PCR&nbsp;Method for Diagnosis of AY-Group Phytoplasmas.-&nbsp;One-step Multiplex Quantitative RT-PCR for the Simultaneous Detection of Viroids and Phytoplasmas.-&nbsp;A Rapid Protocol of Crude RNA/DNA Extraction for RT-qPCR Detection and Quantification.-&nbsp;Quantitative Analysis with Droplet Digital PCR.-&nbsp;Rapid Sample Preparation and LAMP for Phytoplasma Detection.-&nbsp;Assembly of Phytoplasma Genome Drafts from Illumina Reads using Phytoassembly.- Protocol for the Definition of a Multi-Spectral Sensor for Specific Foliar Disease Detection: Case of ‘Flavescence Dorée’.-&nbsp;Transcriptomic Analyses of Phytoplasmas.-&nbsp;Transcriptomic Analyses of Phytoplasmas.-&nbsp;Laser Microdissection of Phytoplasma-infected Grapevine Leaf Phloem Tissue for Gene Expression Study.-&nbsp;Collection of Phloem Sap in Phytoplasma-infected Plants.-&nbsp;DAPI and Confocal Laser-Scanning Microscopy for in vivo Imaging of Phytoplasmas.-&nbsp;Immunofluorescence Assay to Study Early Events of Vector Salivary Gland Colonization by Phytoplasmas.- Characterization of Phytoplasmal Effector Protein Interaction with Proteinaceous Plant Host Targets using Bimolecular Fluorescence Complementation (BiFC).-&nbsp;Collection, Identification, and Statistical Analysis of Volatile Organic Compound Patterns Emitted by Phytoplasma Infected Plants.-&nbsp;Quantification of Phytohormones by HPLC-MS/MS Including Phytoplasma-infected Plants.&nbsp;</p>