Brain-Derived Neurotrophic Factor (BDNF)

<div>Brain Derived Neurotrophic Factor and the “Attività plastic dei neuroni”, the Neuronal Plasticity as Defined by Ernesto Lugaro (1870-1940).-&nbsp;Usage of Bacterial Artificial Chromosomes for Studying BDNF Gene Regulation in Primary Cultures of Cortical Neurons and Astrocytes.-&nbsp;Detecting Single and Multiple BDNF Transcripts In Situ in Neuronal Cultures and Brain Sections.- Studying BDNF/TrkB Signaling: Transcriptome Analysis from a Limited Number of Purified Adult or Aged Murine Brain Neurons.-&nbsp;Studying BDNF/TrkB Signaling: High-Throughput Microfluidic Gene Expression Analysis from rare of Limited Samples of Adult and Aged Central Neurons.-&nbsp;Detecting BDNF Protein Forms by ELISA, Western Blot, and Immunofluorescence.-&nbsp;Methodology for Detecting and Tracking brain-Derived Neurotrophic Factor Complexes in Neurons using Single Quantum Dots.-&nbsp;Recording Activity-Dependent Release of BDNF from Hippocampal Neurons.-&nbsp;Ultrastructural Localization of BDNF and trkB Receptors.-&nbsp;Analysis of TrkB Receptor Activity using FRET Sensors.-&nbsp;BDNF-Induced Intracellular Signaling.-&nbsp;A Microfluidic Culture Platform for Neurotrophin Signaling Studies.-&nbsp;BDNF-Induced Local Protein Synthesis in Synaptoneurosomes Assessed with Click-iT L-azidohomoalanine.-&nbsp;Proteomic Tools to Study the Effect of BDNF on de novo Protein Synthesis.-&nbsp;BDNF Function in Long-Term Synaptic Plasticity in the Dentate Gyrus in Vivo: Methods for Local Drug Delivery and Biochemical Analysis of Translation.</div>