Plant MicroRNAs

<p>1. An Introduction to Methods for Discovery and Functional Analysis of MicroRNAs in Plants</p>

<p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Alma Armenta-Medina and C. Stewart Gillmor</p>

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<p>2. The Plant MicroRNA Pathway: The Production and Action Stages</p>

<p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Joseph L. Pegler, Christopher P. Grof, and Andrew L. Eamens</p>

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<p>3. Plant miRNA Conservation and Evolution</p>

<p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Lydia Gramzow and Günter Theißen</p>

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4. Bioinformatic Analysis of Small RNA Sequencing Libraries<p></p>

<p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Ricardo A. Chávez Montes, Fabiola Jaimes Miranda, and Stefan de Folter</p>

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<p>5. An Overview of miRNA and miRNA Target Analysis Tools</p>

<p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Anna Lukasik and Piotr Zielenkiewicz</p>

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<p>6. miRLocator: A Python Implementation and Web Server for Predicting miRNAs from Pre-miRNA Sequences</p>

<p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Ting Zhang, Lie Ju, Jingjing Zhai, Yujia Song, Jie Song, and Chuang Ma</p>

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<p>7. Prediction of Plant miRNA Targets</p>

<p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Priyanka Pandey, Prashant K. Srivastava, and Shree P. Pandey</p>

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<p>8. Isolation and Detection Methods of Plant miRNAs</p>

<p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Pedro Fernando Vera Hernández, Stefan de Folter, and Flor de Fátima Rosas Cárdenas</p>

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<p>9. Northern Blot Analysis of MicroRNAs and Other Small RNAs in Plants</p>

<p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Carlos De la Rosa and José Luis Reyes</p>

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<p>10. miRNA Detection by Stem-Loop RT-qPCR in Studying MicroRNA Biogenesis and MicroRNA Responsiveness to Abiotic Stresses</p>

<p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Aleksandra Smoczynska, Pawel Sega, Agata Stepien, Katarzyna Knop, Artur Jarmolowski, Andrzej Pacak, and Zofia Szweykowska-Kulinska</p>

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<p>11. Detection of miRNAs by Tissue Printing and Dot Blot Hybridization</p>

<p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Marcelino Martínez Núñez, Stefan de Folter, and Flor de Fátima Rosas Cárdenas</p>

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<p>12. In Situ Localization of Small RNAs in Plants</p>

<p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Cristina F. Marco, Damianos S. Skopelitis, and Marja C.P. Timmermans</p>

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<p>13. Expression Pattern of Plant miRNAs by Classical Transcriptional Fusion Constructs</p>

<p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Andrea Tovar-Aguilar, Karla A. Sánchez-Elizondo, Alejandra Rodríguez-Rodríguez, Marcos I. González-Jaime, Genaro Patino-Lopez, Vadim Perez-Koldenkova, Jesus A. Badillo-Corona, and Noé Durán-Figueroa</p>

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<p>14. A Simple Protocol for Imaging Floral Tissues of Arabidopsis with Confocal Microscopy</p>

<p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Andrea Gómez-Felipe and Stefan de Folter</p>

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<p>15. Degradome Sequencing in Plants</p>

<p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Shih-Shun Lin, Yihua Chen, and Mei-Yeh Jade Lu</p>

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<p>16. Functional Analysis of Root MicroRNAs by a Constitutive Overexpression Approach in a Composite Plant System</p>

<p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Damien Formey, José Ángel Martín-Rodríguez, and Georgina Hernández</p>

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<p>17. The Use of MicroRNA Decoy Technologies to Inhibit miRNA Function in Arabidopsis</p>

<p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Gigi Wong and Anthony A. Millar</p>

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<p>18. Mimicry Technology: A Versatile Tool for Small RNA Suppression</p>

&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Luis Manuel Villar-Martin and Ignacio Rubio-Somoza<p></p>

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<p>19. Design and High-Throughput Generation of Artificial Small RNA Constructs for Plants</p>

<p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Alberto Carbonell</p>

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<p>20. Detection of MicroRNA Processing Intermediates through RNA Ligation Approaches</p>

<p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Belen Moro, Arantxa M.L Rojas, and Javier F. Palatnik</p>

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<p>21. Analysis of Methylation Status of Plant MicroRNAs</p>

<p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Susu Chen and Guodong Ren</p>

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<p>22. Detection of Slicer Activity by Immunopurified Plant ARGONAUTE1</p>

<p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Laura Arribas-Hernández, Maria Louisa Vigh, and Peter Brodersen</p>

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<p>23. The Isolation of Total and Membrane-Bound Polysomes from Arabidopsis and the Detection of Their Associated AGO1 and sRNAs</p>

<p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Shengben Li</p>

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<p>24. Immunolocalization to Study ARGONAUTE Proteins in Developing Ovules of the Brassicaceae</p>

<p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Gloria León-Martínez, Edgar Demesa-Arévalo, and Jean-Philippe Vielle-Calzada</p>

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<p>25. Detection of Histone Modifications Associated with miRNAs</p>

<p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Sara Hernández-Castellano and Clelia De-la-Peña</p>