Plant MicroRNAs

An Introduction to Methods for Discovery and Functional Analysis of MicroRNAs in Plants.- The Plant MicroRNA Pathway: The Production and Action Stages.- Plant miRNA Conservation and Evolution.- Bioinformatic Analysis of Small RNA Sequencing Libraries.- An Overview of miRNA and miRNA Target Analysis Tools.- miRLocator: A Python Implementation and Web Server for Predicting miRNAs from Pre-miRNA Sequences.- Prediction of Plant miRNA Targets.- Isolation and Detection Methods of Plant miRNAs.- Northern Blot Analysis of MicroRNAs and Other Small RNAs in Plants.- miRNA Detection by Stem-Loop RT-qPCR in Studying MicroRNA Biogenesis and MicroRNA Responsiveness to Abiotic Stresses.- Detection of miRNAs by Tissue Printing and Dot Blot Hybridization.- In Situ Localization of Small RNAs in Plants.- Expression Pattern of Plant miRNAs by Classical Transcriptional Fusion Constructs.- A Simple Protocol for Imaging Floral Tissues of Arabidopsis with Confocal Microscopy.- Degradome Sequencing in Plants.-Functional Analysis of Root MicroRNAs by a Constitutive Overexpression Approach in a Composite Plant System.- The Use of MicroRNA Decoy Technologies to Inhibit miRNA Function in Arabidopsis.- Mimicry Technology: A Versatile Tool for Small RNA Suppression.- Design and High-Throughput Generation of Artificial Small RNA Constructs for Plants.- Detection of MicroRNA Processing Intermediates through RNA Ligation Approaches.- Analysis of Methylation Status of Plant MicroRNAs.- Detection of Slicer Activity by Immunopurified Plant ARGONAUTE1.- The Isolation of Total and Membrane-Bound Polysomes from Arabidopsis and the Detection of Their Associated AGO1 and sRNAs.- Immunolocalization to Study ARGONAUTE Proteins in Developing Ovules of the Brassicaceae.- Detection of Histone Modifications Associated with miRNAs.