Legionella

Analysis of Iron Requirements and Siderophore Production.- The Pathometabolism of Legionella Studied by Isotopologue Profiling.- Quantification of Viable but Non-culturable Cells of Legionella pneumophila.- Selection of Legionella Virulence-related Traits by Environmental Protozoa.- Migration of Acanthamoeba castellanii through Legionella Biofilms.- Scar-free Genome Editing in Legionella pneumophila.- Querying Legionella Genomes using Transposition-sequencing.- Screening Targeted Legionella pneumophila Mutant Libraries in vivo using INSeq.- Genetic Manipulation of non-pneumophila Legionella: Protocols Developed for Legionella longbeachae.- Quantitative Imaging Flow Cytometry of Legionella-containing Vacuoles in Dually Fluorescence-labelled Dictyostelium.- Sorting of Phagocytic Cells Infected with Legionella pneumophila.- Single Cell Analysis of Legionella and Legionella-infected Acanthamoeba by Agarose Embedment.- Mitochondrial Dynamics and Activity in Legionella-Infected Cells.- Perturbation of Legionella Cell Infection by RNA Interference.- Isolation of the Dot/Icm Type IV Secretion System Core Complex from Legionella pneumophila.- In situ Imaging and Structure Determination of Bacterial Toxin Delivery Systems using Electron Cryotomography.- Methods for Non-canonical Ubiquitination and Deubiquitination Catalyzed by Legionella pneumophila Effector Proteins.- Purification and Analysis of Effector Glucosyltransferase Lgt1 from Legionella pneumophila.- Determination of in vivo Interactomes of Dot/Icm type IV Secretion System Effectors by Tandem Affinity Purification.- Inflammasome Activation in Legionella-infected Macrophages.- Infection of Human Lung Tissue Explants (HLTEs) with Legionella pneumophila.- The Galleria mellonella Infection Model for Investigating the Molecular Mechanisms of Legionella Virulence.- Dictyostelium Host Response to Legionella Infection: Strategies and Assays.- 6.1.3 The Caenorhabditis elegans model of Legionella infection.- The Mouse as a Model for Pulmonary Legionella Infection.- Targeted Metagenomics of Microbial Diversity in Free-living Amoebae and Water Samples.- Analyses of the Pulmonary Microbiome Composition of Legionella Pneumophila-Infected Patients.- Subcellular Protein Fractionation in Legionella pneumophila and Preparation of the Derived Sub-proteomes for Analysis by Mass Spectrometry.