G Protein-Coupled Receptor Signaling

Assessment of Conformational State Transitions of Class B GPCRs Using Molecular Dynamics.- Molecular Dynamic Simulations to Probe Water Permeation Pathways of GPCRs.- Expression and Purification of a Functional E. coli ¹³CH₃-Methionine-Labeled Thermostable Neurotensin Receptor 1 Variant for Solution NMR Studies.- A Combined Cell-Free Protein Synthesis and Fluorescence-Based Approach to Investigate GPCR Binding Properties.- Furan Cross-Linking Technology for Investigating GPCR-Ligand Interactions.- Optical Regulation of Class C GPCRs by Photoswitchable Orthogonal Remotely-Tethered Ligands.- Chemoselective Acylation of Hydrazinopeptides to Access Fluorescent Probes for Time-Resolved FRET Assay on GPCRs.- Time-Resolved FRET-Based Assays to Characterize G Protein-Coupled Receptor Hetero-Oligomer Pharmacology.- Combining Conformational Profiling of GPCRs with CRISPR/Cas9 Gene Editing Approaches.- Measuring GPCR Stoichiometry Using Types -1, -2 and -3 Bioluminescence Resonance Energy Transfer-Based Assays.- Combining SRET2 and BiFC to Study GPCR Heteromerization and Protein-Protein Interactions.- Quantification and Comparison of Signals Generated by Different FRET-Based cAMP Reporters.- Measuring GPCR-Induced Activation of Protein Tyrosine Phosphatases (PTP) Using In-Gel and Colorimetric PTP Assays.- Measurement of β-Arrestin Recruitment at GPCRs Using the Tango Assay.- Probing the Interactome of Corticotropin-Releasing Factor Receptor Heteromers Using Mass Spectrometry.- Monitoring the Aggregation of GPCRs by Fluorescence Microscopy.- Combining RNAi and Immunofluorescence Approaches to Investigate Post-Endocytic Sorting of GPCRs into Multivesicular Bodies.- Super-Resolution Imaging of G Protein-Coupled Receptors Using Ground State Depletion Microscopy.- Analysis of Spatial Assembly of GPCRs Using Photoactivatable Dyes and Localization Microscopy.- Optical Modulation of Metabotropic Glutamate Receptor Type 5 In Vivo Using a Photoactive Drug.- Imaging of Tissue-Specific and Temporal Activation of GPCR Signaling Using DREADD Knock-In Mice.- Laser Doppler Flowmetry to Study the Regulation of Cerebral Blood Flow by G Protein-Coupled Receptors in Rodents.- Preparation of Pulmonary Artery Myocytes and Rings to Study Vasoactive GPCRs.