Carlos M. Palmeira,
António J. Moreno
Mitochondrial Bioenergetics
Methods and Protocols
Paperback Engels 2019 2e druk 9781493992980Levertijd ongeveer 9 werkdagen
Gratis verzonden
Samenvatting
This second edition volume expands on the previous edition with chapters discussing the latest developments and research initiatives in mitochondrial functions. The chapters in this book explore topics such as high-resolution fluorespirometry and OXPHOS protocols in human cells, analysis of mitochondrial oxygen consumption, mitochondrial bioenergetics, and mitochondrial dynamics in mammalian cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and practical, Mitochondrial Bioenergetics: Methods and Protocols, Second Edition is a valuable resource for students, and practitioners in the pharmaceutical sciences, environmental sciences, and mitochondrial genetics industries.
Specificaties
ISBN13:9781493992980
Taal:Engels
Bindwijze:paperback
Uitgever:Springer New York
Druk:2
Lezersrecensies
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Inhoudsopgave
Preface…Table of Contents…Contributing Authors…
1. Overview of Mitochondrial BioenergeticsVitor M.C. Madeira
2. Evaluation of Respiration with Clark Type Electrode in Isolated Mitochondria and Permeabilized Animal CellsAna M. Silva and Paulo J. Oliveira
3. High-Resolution FluoRespirometry and OXPHOS Protocols for Human Cells, Permeabilized Fibres from Small Biopsies of Muscle and Isolated MitochondriaCarolina Doerrier, Luiz F. Garcia-Souza, Gerhard Krumschnabel, Yvonne Wohlfarter, András T. Mészáros, and Erich Gnaiger
4. High-Throughput Analysis of Mitochondrial Oxygen ConsumptionJames Hynes, Rachel L. Swiss, and Yvonne Will
5. Modulation of Cellular Respiration by Endogenously Produced Nitric Oxide in Rat Hippocampal SlicesAna Ledo, Rui M. Barbosa, and João Laranjinha
6. Mitochondrial Membrane Potential (ΔΨ) Fluctuations Associated with the Metabolic States of MitochondriaJoão S. Teodoro, Anabela P. Rolo, and Carlos M. Palmeira
7. Fluorescence Measurement of Mitochondrial Membrane Potential Changes in Cultured CellsDavid G. Nicholls
8. Comparison of Mitochondrial Incubation Media for Measurement of Respiration and Hydrogen Peroxide ProductionTimea Komlódi, Ondrej Sobotka, Gerhard Krumschnabel, Nicole Bezuidenhout, Elisabeth Hiller, Carolina Doerrier, and Erich Gnaiger
9. Measurement of Proton Leak in Isolated MitochondriaCharles Affourtit, Hoi-Shan Wong, and Martin D. Brand
10. Imaging Mitochondrial Calcium Fluxes with Fluorescent Probes and Single or Two Photon Confocal MicroscopySean M. Davidson and Michael R. Duchen
11. Mitochondrial Permeability Transition Pore and Calcium HandlingRandi J. Parks, Elizabeth Murphy, and Julia C. Liu
12. Redox Equivalents and Mitochondrial BioenergeticsJames R. Roede, Young-Mi Go, and Dean P. Jones
13. Mitochondrial Bioenergetics by 13 C-NMR Isotopomer AnalysisRui A. Carvalho
14. Computational Modeling of Mitochondrial Function from a Systems Biology PerspectiveSonia Cortassa, Steven J. Sollott, and Miguel A. Aon
15. Monitoring the Mitochondrial Dynamics in Mammalian CellsLuca Simula and Silvia Campello
16. Plate-Based Measurement of Superoxide and Hydrogen Peroxide Production by Isolated MitochondriaHoi-Shan Wong, Pierre-Axel Monternier, Adam L. Orr, and Martin D. Brand
17. Plate-Based Measurement of Respiration by Isolated MitochondriaShona A. Mookerjee, Casey L. Quinlan, Helen Wong, Pratiksha Dighe, and Martin D. Brand
18. Detection of Iron Depletion- and Hypoxia-Induced Mitophagy in Mammalian CellsShun-ichi Yamashita and Tomotake Kanki
19. The Importance of Calcium Ions for Determining Mitochondrial Glycerol-3-Phosphate Dehydrogenase Activity When Measuring Uncoupling Protein 1 (UCP1) Function in Mitochondria Isolated from Brown Adipose TissueKieran J. Clarke and Richard K. Porter
20. Isolation and Analysis of Mitochondrial Small RNAs from Rat Liver Tissue and HepG2 CellsJulian Geiger and Louise T. Dalgaard
21. Imaging of Mitochondrial pH Using SNARF-1Venkat K. Ramshesh and John J. Lemasters
22. Relation between Mitochondrial Membrane Potential and ROS FormationJan Suski, Magdalena Lebiedzinska, Massimo Bonora, Paolo Pinton, Jerzy Duszynski, and Mariusz R. Wieckowski
23. Assessing Spatio-Temporal and Functional Organization of Mitochondrial NetworksFelix T. Kurz, Miguel A. Aon, Brian O’Rourke and Antonis A. Armoundas
24. Measurement of Mitochondrial ROS FormationSoni Deshwal, Salvatore Antonucci, Nina Kaludercic, and Fabio Di Lisa
1. Overview of Mitochondrial BioenergeticsVitor M.C. Madeira
2. Evaluation of Respiration with Clark Type Electrode in Isolated Mitochondria and Permeabilized Animal CellsAna M. Silva and Paulo J. Oliveira
3. High-Resolution FluoRespirometry and OXPHOS Protocols for Human Cells, Permeabilized Fibres from Small Biopsies of Muscle and Isolated MitochondriaCarolina Doerrier, Luiz F. Garcia-Souza, Gerhard Krumschnabel, Yvonne Wohlfarter, András T. Mészáros, and Erich Gnaiger
4. High-Throughput Analysis of Mitochondrial Oxygen ConsumptionJames Hynes, Rachel L. Swiss, and Yvonne Will
5. Modulation of Cellular Respiration by Endogenously Produced Nitric Oxide in Rat Hippocampal SlicesAna Ledo, Rui M. Barbosa, and João Laranjinha
6. Mitochondrial Membrane Potential (ΔΨ) Fluctuations Associated with the Metabolic States of MitochondriaJoão S. Teodoro, Anabela P. Rolo, and Carlos M. Palmeira
7. Fluorescence Measurement of Mitochondrial Membrane Potential Changes in Cultured CellsDavid G. Nicholls
8. Comparison of Mitochondrial Incubation Media for Measurement of Respiration and Hydrogen Peroxide ProductionTimea Komlódi, Ondrej Sobotka, Gerhard Krumschnabel, Nicole Bezuidenhout, Elisabeth Hiller, Carolina Doerrier, and Erich Gnaiger
9. Measurement of Proton Leak in Isolated MitochondriaCharles Affourtit, Hoi-Shan Wong, and Martin D. Brand
10. Imaging Mitochondrial Calcium Fluxes with Fluorescent Probes and Single or Two Photon Confocal MicroscopySean M. Davidson and Michael R. Duchen
11. Mitochondrial Permeability Transition Pore and Calcium HandlingRandi J. Parks, Elizabeth Murphy, and Julia C. Liu
12. Redox Equivalents and Mitochondrial BioenergeticsJames R. Roede, Young-Mi Go, and Dean P. Jones
13. Mitochondrial Bioenergetics by 13 C-NMR Isotopomer AnalysisRui A. Carvalho
14. Computational Modeling of Mitochondrial Function from a Systems Biology PerspectiveSonia Cortassa, Steven J. Sollott, and Miguel A. Aon
15. Monitoring the Mitochondrial Dynamics in Mammalian CellsLuca Simula and Silvia Campello
16. Plate-Based Measurement of Superoxide and Hydrogen Peroxide Production by Isolated MitochondriaHoi-Shan Wong, Pierre-Axel Monternier, Adam L. Orr, and Martin D. Brand
17. Plate-Based Measurement of Respiration by Isolated MitochondriaShona A. Mookerjee, Casey L. Quinlan, Helen Wong, Pratiksha Dighe, and Martin D. Brand
18. Detection of Iron Depletion- and Hypoxia-Induced Mitophagy in Mammalian CellsShun-ichi Yamashita and Tomotake Kanki
19. The Importance of Calcium Ions for Determining Mitochondrial Glycerol-3-Phosphate Dehydrogenase Activity When Measuring Uncoupling Protein 1 (UCP1) Function in Mitochondria Isolated from Brown Adipose TissueKieran J. Clarke and Richard K. Porter
20. Isolation and Analysis of Mitochondrial Small RNAs from Rat Liver Tissue and HepG2 CellsJulian Geiger and Louise T. Dalgaard
21. Imaging of Mitochondrial pH Using SNARF-1Venkat K. Ramshesh and John J. Lemasters
22. Relation between Mitochondrial Membrane Potential and ROS FormationJan Suski, Magdalena Lebiedzinska, Massimo Bonora, Paolo Pinton, Jerzy Duszynski, and Mariusz R. Wieckowski
23. Assessing Spatio-Temporal and Functional Organization of Mitochondrial NetworksFelix T. Kurz, Miguel A. Aon, Brian O’Rourke and Antonis A. Armoundas
24. Measurement of Mitochondrial ROS FormationSoni Deshwal, Salvatore Antonucci, Nina Kaludercic, and Fabio Di Lisa
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