The CRAC Channel

<div>Patch-Clamp Recording of the CRAC Channel Current in STIM-Orai Overexpressing Cells.-&nbsp;Fluorescence-Based Ratiometric Measurement of CRAC Channel Activity in STIM-Orai Overexpressing HEK-293 Cells.-&nbsp;Recording SOCE Activity in Neurons by Patch-Clamp Electrophysiology and Microfluorometric Calcium Imaging.-&nbsp;Mn2+ Quenching Assay for Store-Operated Calcium Entry.-&nbsp;Fluorescence-Based Measurements of Store-Operated Ca2+ Entry in Cancer Cells using Fluo-4 and Confocal Live Cell Imaging.-&nbsp;Fluorescence-Based Measurements of the CRAC Channel Activity in Cell Populations.-&nbsp;Indirect Measurement of CRAC Channel Activity using NFAT Nuclear Translocation by Flow Cytometry in Jurkat Cells.-&nbsp;CRAC Channel&nbsp; Components Quantitative Expression (In Tissues and Cell Lines) using qPCR.-&nbsp;Western-Blotting and Co-Immunoprecipitation of Endogenous STIM/ORAI and Protein Partners.-&nbsp;Study of Endogenous CRAC Channels in Human Mast Cells using an Adenoviral Delivery System to Transduce Cells with Orai-Targeting shRNAs or with cDNAs Expressing Dominant-Negative Orai Channel Mutations.-&nbsp;Store-Operated Ca2+ Entry in Drosophila Primary Neuron Cultures.-&nbsp;Study of the Endogenous CRAC Channel using shRNA-Mediated Gene Silencing.-&nbsp;Engineered Cross-Linking to Study the Pore Architecture of the CRAC Channel.-&nbsp;Measurement of the CRAC Channel Fast Ca2+-Dependent Inactivation (FCDI).-&nbsp;High Resolution Imaging of STIM/Orai Subcellular Localization using Array Confocal Laser Scanning Microscopy.-&nbsp;Single Channel Single Molecule Detection (SC-SMD) System.</div>