Medical BioMethods Handbook

Basic Techniques in Molecular Biology.- Probe Design, Production, and Applications.- Restriction Enzymes.- Southern Blotting as a Diagnostic Method.- Western Blotting as a Diagnostic Method.- Principles and Medical Applications of the Polymerase Chain Reaction.- Single-Strand Conformation Polymorphism (SSCP) Analysis.- Denaturing Gradient Gel Electrophoresis (DGGE).- Quantitative Analysis of DNA Sequences by PCR and Solid-Phase Minisequencing.- to Capillary Electrophoresis of DNA.- Mapping Techniques.- Dideoxyfingerprinting for Mutation Detection.- Conformation-Sensitive Gel Electrophoresis.- Amplification Refractory Mutation System and Molecular Diagnostics.- Ligase Chain Reaction.- Chemical Cleavage of Mismatch.- The Protein Truncation Test.- Linkage, Allele Sharing, and Association.- Single-Nucleotide Polymorphisms.- cDNA Microarrays.- Nucleic Acid Sequence-Based Amplification.- Oligonucleotide Ligation Assay.- Quantitative TaqMan Real-Time PCR.- Use of Denaturing High-Performance Liquid Chromatography in Molecular Medicine.- Quantitative PCR.- Liquid Chromatography-Mass Spectrometry of Nucleic Acids.- Comparative Genomic Hybridization in Clinical and Medical Research.- Bioinformatic Tools for Gene and Protein Sequence Analysis.- In SituHybridization.- Enzyme-Linked Immunosorbent Assay.- Protein Therapeutics.- Karyotyping.- Microsatellite Analysis.- Analysis of Chromosomal Translocations.- Differential Display.- Techniques for Gene Expression Profiling.- Capillary Electrophoresis in Clinical Analysis.- Flow Cytometry in the Biomedical Arena.- Immunocytochemistry.- Ribotyping in Clinical Microbiology.- Diagnostic Applications of Protein Microarrays.- The Human Genome Project.- Prenatal Diagnosis of Inborn Errors of Metabolism.- Gene Therapy.