Membrane Trafficking

Yeast Cells.- 1 Freight Management in the Cell: Current Aspects of Intracellular Membrane Trafficking.- High-Throughput Protein Extraction and Immunoblotting Analysis in Saccharomyces cerevisiae.- Genome-Wide Analysis of Membrane Transport Using Yeast Knockout Arrays.- A Cell-Free System for Reconstitution of Transport Between Prevacuolar Compartments and Vacuoles in Saccharomyces cerevisiae.- In vitro Analysis of the Mitochondrial Preprotein Import Machinery Using Recombinant Precursor Polypeptides.- In Vitro Import of Proteins Into Isolated Mitochondria.- Synthesis and Sorting of Mitochondrial Translation Products.- In Vivo Labeling and Analysis of Mitochondrial Translation Products in Budding and in Fission Yeasts.- Exploring Protein-Protein Interactions Involving Newly Synthesized Mitochondrial DNA-Encoded Proteins.- Purification of Yeast Membranes and Organelles by Sucrose Density Gradient Centrifugation.- Microscopic Analysis of Lipid Droplet Metabolism and Dynamics in Yeast.- Use of Bimolecular Fluorescence Complementation in Yeast Saccharomyces cerevisiae.- Mammalian Cells.- Using Quantitative Fluorescence Microscopy to Probe Organelle Assembly and Membrane Trafficking.- Measuring Secretory Membrane Traffic.- A Correlative Light and Electron Microscopy Method Based on Laser Micropatterning and Etching.- Approaches to Investigate the Role of Signaling in ER-to-Golgi Transport.- Recruitment of Coat Proteins to Peptidoliposomes.- SNARE-Mediated Fusion of LIposomes.- Use of Polarized PC12 Cells to Monitor Protein Localization in the Early Biosynthetic Pathway.- Tracking the Transport of E-Cadherin to and From the Plasma Membrane.- Analysis of Nucleocytoplasmic Shuttling of NF?B Proteins in Human Leukocytes.- Imaging pHluorin-Based Probes at Hippocampal Synapses.-Analysis of Receptor Tyrosine Kinase Internalization Using Flow Cytometry.- Measurement of Receptor Endocytosis and Recycling.- Styryl Dye-Based Synaptic Vesicle Recycling Assay in Cultured Cerebellar Granule Neurons.- Use of Quantitative Immunofluorescence Microscopy to Study Intracellular Trafficking: Studies of the GLUT4 Glucose Transporter.- Dissecting GLUT4 Traffic Components in L6 Myocytes by Fluorescence-Based, Single-Cell Assays.- Functional Genetic Analysis of the Mammalian Mitochondrial DNA Encoded Peptides.