Leukemia

An Overview: From Discovery of Candidate Mutations to Disease Modeling and Transformation Mechanisms of Acute Leukemia.- Backtracking of Leukemic Clones to Birth.- Fluorescence In Situ Hybridization (FISH) as a Tool for the Detection of Significant Chromosomal Abnormalities in Childhood Leukaemia.- Specialized Fluorescence In Situ Hybridization (FISH) Techniques for Leukaemia Research.- LDI-PCR: Identification of Known and Unknown Gene Fusions of the Human MLL Gene.- Panhandle PCR Approaches to Cloning MLL Genomic Breakpoint Junctions and Fusion Transcript Sequences.- MRD Detection in Acute Lymphoblastic Leukemia Patients Using Ig/TCR Gene Rearrangements as Targets for Real-Time Quantitative PCR.- Array-Based Comparative Genomic Hybridization as a Tool for Analyzing the Leukemia Genome.- Application of SNP Genotype Arrays to Determine Somatic Changes in Cancer.- Retroviral/Lentiviral Transduction and Transformation Assay.- Gene Expression Profiling of Leukemia Stem Cells.- Humanized Model to Study Leukemic Stem Cells.- Model Systems for Examining Effects of Leukemia Associated Oncogenes in Primary Human CD34+ Cells via Retroviral Transduction.- Ex Vivo Assays to Study Self-Renewal and Long-Term Expansion of Genetically Modified Primary Human Acute Myeloid Leukemia Stem Cells.- Identification and Characterization of Hematopoietic Stem and Progenitor Cell Populations in Mouse Bone Marrow by Flow Cytometry.- In Vitro Differentiation of Embryonic Stem Cells as a Model of Early Hematopoietic Development.- Analysis of the Mouse Placenta as a Hematopoietic Stem Cell Niche.- Identification of Protein Interaction Partners by the Yeast Two-Hybrid System.- Complete Array of HOX Gene Expression by RQ-PCR.- Genome-Wide Determination of DNA Methylation by Hpa II Tiny Fragment Enrichment by Ligation-Mediated PCR (HELP) for the Study of Acute Leukemias.- Chromatin Immunoprecipitation (ChIP) for Analysis of Histone Modifications and Chromatin-Associated Proteins.