PCR Protocols

Setup of a PCR Laboratory.- Long Range PCR with a DNA Polymerase Fusion.- Isolation of Genomic Insertion Sites of Proviruses Using Splinkerette-PCR-Based Procedures.- Lariat-Dependent Nested PCR for Flanking Sequence Determination.- CODEHOP PCR and CODEHOP PCR Primer Design.- Sequencing of Difficult DNA Regions by SAM Sequencing.- A Global Single-Cell cDNA Amplification Method for Quantitative Microarray Analysis.- Quantitation of MicroRNAs by Real-Time RT-qPCR.- High-Throughput SuperSAGE.- Deep Cap Analysis of Gene Expression (Deep CAGE).- Linking Emulsion PCR Haplotype Analysis.- PAP-LMPCR: An Improved, Sequence-Selective Method for the In vivo Analysis of Transcription Factor Occupancy and Chromatin Fine Structure.- The Many Faces of MLPA.- Assessing Gene-Specific Methylation Using HRM-Based Analysis.- Alu PCR.- Asynchronous PCR.- Novel Applications of PCR through the Use of DNA Substrates.- Enhanced Solid Phase PCR for Increased Loading of Amplicon onto Solid Support.- Application of Blocking Oligonucleotides to Improve Signal to Noise Ratio in a PCR.- Asymmetric Overlap Extension PCR Method for Site-Directed Mutagenesis.- Ribosome Display: A Technology for Selecting and Evolving Proteins from Large Libraries.- GLOBE: Analysis of DNA-Protein Interaction Analysis.- PCR DNA-Array Profiling of DNA-Binding Transcription Factor Activities in Adult Mouse Tissues.- Nucleotide Exchange and Excision Technology (NExT) DNA Shuffling and Directed Evolution.