DNA'Protein Interactions
Principles and Protocols
Paperback Engels 2011 2e druk 9781617371257Samenvatting
Dr. Tom Moss assembles the new standard collection of cutting-edge techniques to identify key protein-DNA interactions and define their components, their manner of interaction, and their manner of function, both in the cell and in the test tube. The techniques span a wide range, from factor identification to atomic detail, and include multiple DNA footprinting analyses, including in vivo strategies, gel shift (EMSA) optimization, SELEX, surface plasmon resonance, site-specific DNA-protein crosslinking, and UV laser crosslinking. Comprehensive and broad ranging, DNA-Protein Interactions: Principles and Protocols, 2nd Edition, offers a stellar array of over 100 up-to-date and readily reproducible techniques that biochemists and molecular, cellular, and developmental biologists can use successfully today to understand DNA-protein interactions.
Specificaties
Lezersrecensies
Inhoudsopgave
Peter G. Stockley<BR>
<BR>
Electrophoretic Mobility Shift Assays for the Analysis of DNA-Protein Interactions<BR>
Marc-André Laniel, Alain Béliveau, and Sylvain L. Guérin<BR>
<BR>
DNase I Footprinting, Benoît Leblanc and Tom Moss. Footprinting with Exonuclease III<BR>
Willi Metzger and Hermann Heumann<BR>
<BR>
Hydroxyl Radical Footprinting<BR>
Evgeny Zaychikov, Peter Schickor, Ludmilla Denissova, and Hermann Heumann<BR>
<BR>
The Use of Diethyl Pyrocarbonate and Potassium Permanganate as Probes for Strand Separation and Structural Distortions in DNA<BR>
Brenda F. Kahl and Marvin R. Paule<BR>
<BR>
Footprinting DNA-Protein Interactions in Native Polyacrylamide Gels by Chemical Nucleolytic Activity of 1,10 -Phenanthroline-Copper<BR>
Athanasios G. Papavassilou<BR>
<BR>
Uranyl Photofootprinting<BR>
Peter E. Nielsen<BR>
<BR>
Osmium Tetroxide Modification and the Study of DNA-Protein Interactions<BR>
James A. McClellan<BR>
<BR>
Determination of a Transcription Factor-Binding Site by Nuclease Protection Footprinting onto Southwestern Blots<BR>
Athanasios G. Papavassilou<BR>
<BR>
Diffusible Singlet Oxygen as a Probe of DNA Deformation<BR>
Malcolm Buckle and Andrew A. Travers<BR>
<BR>
Ultraviolet-Laser Footprinting<BR>
Johannes Geiselmann and Frederic Boccard<BR>
<BR>
In Vivo DNA Analysis<BR>
Régen Drouin, Jean-Philippe Therrien, Martin Angers, and Stéphane Ouellet<BR>
<BR>
Identification of Protein-DNA Contacts with Dimethyl Sulfate: Methylation Protection and Methylation Interference<BR>
Peter E. Shaw and A. Francis Stewart<BR>
<BR>
Ethylation Interference<BR>
Iain W. Manfield and Peter G. Stockley<BR>
<BR>
Hydroxyl Radical Interference<BR>
Peter Schickor, Evgeny Zaychiko, and Hermann Heumann<BR>
<BR>
Identification of Sequence-Specific DNA-Binding Proteins by Southwestern Blotting<BR>
Simon Labbé, Gale Stewart, Olivier LaRochelle, Guy G. Poirier, and Carl Séguin<BR>
<BR>
A Competition Assay for DNA Binding Using the Fluorescent Probe ANS<BR>
Ian Taylor and G. Geoff Kneale<BR>
<BR>
Site-Directed Cleavage of DNA by Linker Histone Protein-Fe(II) EDTA Conjugates<BR>
David R. Chafin and Jeffrey J. Hayes<BR>
<BR>
Nitration of Tyrosine Residues in Protein-Nucleic Acid Complexes<BR>
Simon E. Plyte<BR>
<BR>
Chemical Modification of Lysine by Reductive Methylation: A Probe of Residues Involved in DNA Binding<BR>
Ian A. Taylor and Michelle Webb<BR>
<BR>
Limited Proteolysis of Protein-Nucleic Acid Complexes<BR>
Simon E. Plyte and G. Geoff Kneale<BR>
<BR>
Ultraviolet Crosslinking of DNA-Protein Complexes Via 8-Azidoadenine<BR>
Rainer Meffert, Klaus Dose, Gabriele Rathgeber, and Hans-Jochen Schäfer<BR>
<BR>
Site-Specific Protein-DNA Photocrosslinking: Analysis of Bacterial Transcription Initiation Complexes<BR>
Nikolai Naryshkin, Younggyu Kim, Qianping Dong, and Richard H. Ebright<BR>
<BR>
Site-Directed DNA Photoaffinity Labeling of RNA Polymerase II Transcription Complexes<BR>
Jim Persinger and Blaine Bartholomew<BR>
<BR>
Use of Site-Specific Protein-DNA Photocrosslinking to Analyze the Molecular Organization of the RNA Polymerase II Initiation Complex<BR>
François Robert and Benoît Coulombe<BR>
<BR>
UV Laser-Induced Protein-DNA Crosslinking<BR>
Stefan I. Dimitrov and Tom Moss<BR>
<BR>
Plasmid Vectors for the Analysis of Protein-Induced DNA Bending<BR>
Christian Zwieb and Sankar Adhya<BR>
<BR>
Engineering Nucleic Acid-Binding Proteins by Phage Display<BR>
Mark Isalan and Yen Choo<BR>
<BR>
Genetic Analysis of DNA-Protein Interactions Using a Reporter Gene Assay in Yeast<BR>
David R. Setzer, Deborah B. Schulman, and Michael J. Bumbulis<BR>
<BR>
Assays for Transcription Factor Activity<BR>
Virgil Rhodius, Nigel Savery, Annie Kolb, and Stephen Busby<BR>
<BR>
Assay of Restriction Endonucleases Using Oligonucleotides<BR>
Bernard A. Connolly, Hsiao-Hui Liu, Damian Parry, Lisa E. Engler, Michael R. Kurpiewski, and Linda Jen-Jacobson<BR>
<BR>
Analysis of DNA-Protein Interactions by Intrinsic Fluorescence<BR>
Mark L. Carpenter, Anthony W. Oliver and G. Geoff Kneale<BR>
<BR>
Circular Dichroism for the Analysis of Protein-DNA Interactions<BR>
Mark L. Carpenter, Anthony W. Oliver, and G. Geoff Kneale<BR>
<BR>
Calorimetry of Protein-DNA Complexes and Their Components<BR>
Christopher M. Read and Ilian Jelesarov<BR>
<BR>
Surface Plasmon Resonance Applied to DNA-Protein Complexes<BR>
Malcolm Buckle<BR>
<BR>
Reconstitution of Protein-DNA Complexes for Crystallization<BR>
Rachel M. Conlin and Raymond S. Brown<BR>
<BR>
Two-Dimensional Crystallization of Soluble Protein Complexes<BR>
Patrick Schultz, Nicolas Bischler, and Luc Lebeau<BR>
<BR>
Atomic Force Microscopy of DNA and Protein-DNA Complexes Using Functionalized Mica Substrates<BR>
Yuri L. Lyubchenko, Alexander A. Gall, and Luda S. Shlyakhtenko<BR>
<BR>
Electron Microscopy of Protein-Nucleic Acid Complexes: Uniform Spreading of Flexible Complexes, Staining with a Uniform Thin Layer of Uranyl Acetate and Determining Helix Handedness<BR>
Carla W. Gray<BR>
<BR>
Scanning Transmission Electon Microscopy of DNA-Protein Complexes<BR>
Joseph S. Wall and Martha N. Simon<BR>
<BR>
Determination of Nuleic Acid Recognition Sequences by SELEX<BR>
Philippe Bouvet<BR>
<BR>
High DNA-Protein Crosslinking Yield with Two-Wavelength Femtosecond Laser Irradiation<BR>
Christoph Russmann, Rene Beigang, and Miguel Beato<BR>
<BR>
Appendices: I. EMSA/Gel Shift Conditions. <BR>
<BR>
II. DNA-Modification/Cleavage Reagents<BR>
<BR>
Index.
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