Drosophila Cytogenetics Protocols

The Chromosomes of Drosophila melanogaster.- Spermatogenesis.- Time-Lapse Imaging of Male Meiosis by Phase-Contrast and Fluorescence Microscopy.- Immunocytological Analysis of Oogenesis.- Cytological Analysis of Oogenesis.- Polytene Chromosomes From Ovarian Nurse Cells of Drosophila melanogaster otu Mutants.- Feulgen-DNA Cytophotometry for Estimating C Values.- Fluorescent BrdU Labeling and Nuclear Flow Sorting of the Drosophila Ovary.- Studying Nuclear Organization in Embryos Using Antibody Tools.- Whole-Mount Fluorescence In Situ Hybridization to Chromosomes of Embryos.- Orcein Staining and the Identification of Polytene Chromosomes.- Salivary Chromosome Analysis of Aberrations.- In Situ Hybridization to Polytene Chromosomes.- Combined Immunostaining and FISH Analysis of Polytene Chromosomes.- Electron Microscopy of Polytene Chromosomes.- Analysis of Mitosis in Squash Preparations of Larval Brains.- Analyzing Chromosome Function by High Frequency Formation of Dicentric Chromosomes In Vivo.- Analysis of Chromosomes of the Larval CNS by FISH and BrdU Labeling.- Immunostaining of Squash Preparations of Chromosomes of Larval Brains.- Visualizing Mitosis in Whole-Mount Larval Brains.- Immunostaining of Whole-Mount Imaginal Discs.- Wing Somatic Mutation and Recombination Test.- Analysis of Histoblasts.- Visualizing Apoptosis.- RNAi in Cultured Drosophila Cells.