Xenopus Protocols

Resources for Genetic and Genomic Studies of Xenopus.- The Physiology of the Xenopus laevis Ovary.- Oocyte Isolation and Enucleation.- Xenopus tropicalis Oocytes.- Oocyte Expression With Injection of Purified T7 RNA Polymerase.- Visualization of the Cytoskeleton in Xenopus Oocytes and Eggs by Confocal Immunofluorescence Microscopy.- Multiphoton Laser Scanning Microscopy as a Tool for Xenopus Oocyte Research.- Imaging Ca2+ Signals in Xenopus Oocytes.- Chromosomal DNA Replication in a Soluble Cell-Free System Derived From Xenopus Eggs.- Chromatin Assembly of DNA Templates Microinjected Into Xenopus Oocytes.- Pre-mRNA Splicing in the Nuclei of Xenopus Oocytes.- Chromatin Immunoprecipitation for Studying Transcriptional Regulation in Xenopus Oocytes and Tadpoles.- Cytoplasmic mRNA Polyadenylation and Translation Assays.- Xenopus Egg Extracts.- Analysis of Molecular Chaperones Using a Xenopus Oocyte Protein Refolding Assay.- Ubiquitin-Mediated Protein Degradation in Xenopus Egg Extracts.- to Nucleocytoplasmic Transport.- Use of Xenopus laevis Oocyte Nuclei and Nuclear Envelopes in Nucleocytoplasmic Transport Studies.- Nuclear Pore Complex Structure and Plasticity Revealed by Electron and Atomic Force Microscopy.- In Vitro Study of Nuclear Assembly and Nuclear Import Using Xenopus Egg Extracts.- Use of Intact Xenopus Oocytes in Nucleocytoplasmic Transport Studies.- Studying the Mechanosensitivity of Voltage-Gated Channels Using Oocyte Patches.- Oocytes as an Expression System for Studying Receptor/Channel Targets of Drugs and Pesticides.- Microtransplantation of Neurotransmitter Receptors From Cells to Xenopus Oocyte Membranes.- Reconstitution of Golgi Disassembly by Mitotic Xenopus Egg Extract in Semi-Intact MDCK Cells.- Exploring RNA Virus Replication in Xenopus Oocytes.- Study of Apoptosis In Vitro Using the Xenopus Egg Extract Reconstitution System.- Studying Fertilization in Cell-Free Extracts.- Localized Sampling, Electrophoresis, and Biosensor Analysis of Xenopus laevis Cytoplasm for Subcellular Biochemical Assays.- Monitoring Protein Kinase A Activities Using Expressed Substrate in Live Cells.- Using Xenopus Oocyte Extracts to Study Signal Transduction.- Oocyte Extracts for the Study of Meiotic M-M Transition.- Methods for Studying Spindle Assembly and Chromosome Condensation in Xenopus Egg Extracts.