Yeast Protocols

Basic Investigations in Saccharomyces cerevisiae.- Isolation of Nucleic Acids.- Purification of Yeast Peroxisomes.- Isolation of Yeast Plasma Membranes.- Isolation of Yeast Mitochondria.- Extraction of Yeast Lipids.- Two-Dimensional Gel Electrophoresis of Total Yeast Proteins.- Pulsed-Field Gel Electrophoresis of Budding Yeast Chromosomes.- Analysis of Yeast Lipids.- Yeast Fluorescence Microscopy.- Intracellular Expression of Recombinant Antibody Fluorescent Protein Fusions for Localization of Target Antigens in Schizosaccharomyces pombe.- Yeast Transformation by the LiAc/SS Carrier DNA/PEG Method.- Mutagenesis.- Gene Disruption in the Budding Yeast Saccharomyces cerevisiae.- Inducible Degron and Its Application to Creating Conditional Mutants.- Synthetic Lethal Screen.- Synthetic Genetic Array Analysis in Saccharomyces cerevisiae.- Two-Dimensional Agarose Gel Analysis of DNA Replication Intermediates.- Chromatin Assembly in a Crude Fraction From Yeast Cells.- Chromatin Immunoprecipitation to Study Protein-DNA Interactions in Budding Yeast.- Isolation of Yeast Nuclei and Micrococcal Nuclease Mapping of Nucleosome Positioning.- Study of Transcriptional Regulation Using a Reporter Gene Assay.- Assessing Telomeric Phenotypes.- Controlled Expression of Recombinant Genes and Preparation of Cell-Free Extracts in Yeast.- Production of Heterologous Proteins in Yeast With the Aid of the Hsp150? Carrier.- Yeast Two-Hybrid System Screening.- Import of Precursor Proteins Into Isolated Yeast Mitochondria.