Ion Channels

Methods for Exogenous Expression of Ion Channels in Cells.- Functional Reconstitution of the Human Epithelial Na+ Channel in a Mammalian Expression System.- Overexpression of Proteins in Neurons Using Replication-Deficient Virus.- Exogenous Expression of Proteins in Neurons Using the Biolistic Particle Delivery System.- Methods for Studying Channel Structure-function.- Tertiary and Quaternary Structure Formation of Voltage-Gated Potassium Channels.- Biophysical Approach to Determine the Subunit Stoichiometry of the Epithelial Sodium Channel Using the Xenopus laevis Oocyte Expression System.- Spectroscopy-Based Quantitative Fluorescence Resonance Energy Transfer Analysis.- Methods for Studying Channel Regulation and Physiological Function.- Probing the Effects of Phosphoinositides on Ion Channels.- Epithelial Sodium Channel in Planar Lipid Bilayers.- A Simple In Vivo Method for Assessing Changes of Membrane-Bound Ion Channel Density in Xenopus Oocytes.- Preparation of Cortical Brain Slices for Electrophysiological Recording.- Juxtacellular Labeling and Chemical Phenotyping of Extracellularly Recorded Neurons In Vivo.- Carbon Fiber Amperometry in the Study of Ion Channels and Secretion.- Methods for Studying Channelopathies, Genetic Screening, and Molecular Biology.- Genetic Screening for Functionality of Bacterial Potassium Channel Mutants Using K+ Uptake-Deficient Escherichia coli.- KCNQ1 K+ Channel—Mediated Cardiac Channelopathies.- Tissue-Specific Transgenic and Knockout Mice.