MicroRNA Protocols

The MicroRNA: Overview of the RNA Gene That Modulates Gene Functions.- Structure Analysis of MicroRNA Precursors.- MicroRNA Biogenesis: Isolation and Characterization of the Microprocessor Complex.- Recognition and Cleavage of Primary MicroRNA Transcripts.- Mouse Embryonic Stem Cells as a Model Genetic System to Dissect and Exploit the RNA Interference Machinery.- MicroRNAs and Messenger RNA Turnover.- Prediction of MicroRNA Targets.- Prediction of Human MicroRNA Targets.- Complications in Mammalian MicroRNA Target Prediction.- miRBase:The MicroRNA Sequence Database.- Methodologies for High-Throughput Expression Profiling of MicroRNAs.- In Situ Hybridization as a Tool to Study the Role of MicroRNAs in Plant Development.- Usefulness of the Luciferase Reporter System to Test the Efficacy of siRNA.- Cloning MicroRNAs From Mammalian Tissues.- Methods for Analyzing MicroRNA Expression and Function During Hematopoietic Lineage Differentiation.- Identifying MicroRNA Regulators of Cell Death in Drosophila.- MicroRNAs in Human Immunodeficiency Virus-1 Infection.- Cloning and Detection of HIV-1-Encoded MicroRNA.- Identification of Messenger RNAs and MicroRNAs Associated With Fragile X Mental Retardation Protein.- In Vitro Precursor MicroRNA Processing Assays Using Drosophila Schneider-2 Cell Lysates.- Downregulation of Human Cdc6 Protein Using a Lentivirus RNA Interference Expression Vector.- Gene Silencing In Vitro and In Vivo Using Intronic MicroRNAs.- Isolation and Identification of Gene-Specific MicroRNAs.- Transgene-Like Animal Models Using Intronic MicroRNAs.- Evolution of MicroRNAs.- Perspectives.