Glycovirology Protocols

Viruses and Glycosylation.- Interaction Between Respiratory Syncytial Virus and Glycosaminoglycans, Including Heparan Sulfate.- Expression of the Severe Acute Respiratory Syndrome Coronavirus 3a Protein and the Assembly of Coronavirus-Like Particles in the Baculovirus Expression System.- The C Type Lectins DC-SIGN and L-SIGN.- Functional Analysis of the N-Linked Glycans Within the Fusion Protein of Respiratory Syncytial Virus.- Expression and Purification of Viral Glycoproteins Using Recombinant Vaccinia Viruses for Functional and Structural Studies.- The Use of Two-Dimensional SDS-PAGE to Analyze the Glycan Heterogeneity of the Respiratory Syncytial Virus Fusion Protein.- The Use of Monoclonal Antibodies and Lectins to Identify Changes in Viral Glycoproteins That are Influenced by Glycosylation.- Expression, Glycosylation, and Modification of the Spike (S) Glycoprotein of SARS CoV.- Analysis of Glycoproteins of Viruses in the Family Bunyaviridae.- Secretion of the Respiratory Syncytial Virus Fusion Protein From Insect Cells Using the Baculovirus Expression System.- Characterization of the Dengue Virus Envelope Glycoprotein Expressed in Pichia pastoris.- Cloning, Expression, and Functional Analysis of Patient-Derived Hepatitis C Virus Glycoproteins.