Bacteriophages

Bacteriophage Isolation.- Methods for the Isolation of Viruses from Environmental Samples.- Bacteriophage Enrichment from Water and Soil.- Isolation of Phage via Induction of Lysogens.- Isolation of Cyanophages from Aquatic Environments.- Isolation of Viruses from High Temperature Environments.- Isolation of Novel Large and Aggregating Bacteriophages.- Bacteriophage Characterization.- Enumeration of Bacteriophages by Double Agar Overlay Plaque Assay.- Enumeration of Bacteriophages by the Direct Plating Plaque Assay.- Enumeration of Bacteriophages Using the Small Drop Plaque Assay System.- Determination of Virus Abundance by Epifluorescence Microscopy.- Enumeration of Bacteriophages Using Flow Cytometry.- Basic Phage Electron Microscopy.- Phage Classification and Characterization.- Phage Host Range and Efficiency of Plating.- Measurement of the Rate of Attachment of Bacteriophage to Cells.- Measurement of the Bacteriophage Inactivation Kinetics with Purified Receptors.- Bacteriophage Plaques: Theory and Analysis.- Practical Methods for Determining Phage Growth Parameters.- Phage Production and Maintenance of Stocks, Including Expected Stock Lifetimes.- Bacteriophage-Host Interactions.- Construction of Phage Mutants.- Modifying Bacteriophage with Recombineering.- Identification and Isolation of Lysogens with Induced Prophage.- Generalized Transduction.- Preparation and Characterization of Anti-phage Serum.- Generalized Transduction by Lytic Bacteriophages.