SARS- and Other Coronaviruses

Detection and Discovery of Coronaviruses.- A Pancoronavirus RT-PCR Assay for Detection of All Known Coronaviruses.- Detection of Group 1 Coronaviruses in Bats Using Universal Coronavirus Reverse Transcription Polymerase Chain Reactions.- Detection and Sequence Characterization of the 3?-End of Coronavirus Genomes Harboring the Highly Conserved RNA Motif s2m.- RT-PCR Detection of Avian Coronaviruses of Galliform Birds (Chicken, Turkey, Pheasant) and in a Parrot.- Detection of Group 2a Coronaviruses with Emphasis on Bovine and Wild Ruminant Strains.- Detection of SARS Coronavirus in Humans and Animals by Conventional and Quantitative (Real Time) Reverse Transcription Polymerase Chain Reactions.- Detection of New Viruses by VIDISCA.- Isolation, Growth, Titration, and Purification of Coronaviruses.- Titration of Human Coronaviruses, HCoV-229E and HCoV-OC43, by an Indirect Immunoperoxidase Assay.- The Preparation of Chicken Tracheal Organ Cultures for Virus Isolation, Propagation, and Titration.- Isolation and Propagation of Coronaviruses inEmbryonated Eggs.- Large-Scale Preparation of UV-Inactivated SARS Coronavirus Virions for Vaccine Antigen.- Structure of Covs Analyzed by Electron Microscopy.- Purification and Electron Cryomicroscopy of Coronavirus Particles.- Expression of Coronavirus Proteins and Crystallization.- Production of Coronavirus Nonstructural Proteins in Soluble Form for Crystallization.- Raising Antibodies to Coronavirus Proteins.- Generating Antibodies to the Gene 3 Proteins of Infectious Bronchitis Virus.- Establishment and Characterization of Monoclonal Antibodies Against SARS Coronavirus.- Production of Monospecific Rabbit Antisera Recognizing Nidovirus Proteins.- Manipulating the Genomes of Coronaviruses.- Manipulation of the Coronavirus GenomeUsing Targeted RNA Recombination with Interspecies Chimeric Coronaviruses.- Generation of Recombinant Coronaviruses Using Vaccinia Virus as the Cloning Vector and Stable Cell Lines Containing Coronaviral Replicon RNAs.- Transient Dominant Selection for the Modification and Generation of Recombinant Infectious Bronchitis Coronaviruses.- Engineering Infectious cDNAs of Coronavirus as Bacterial Artificial Chromosomes.- Systematic Assembly and Genetic Manipulation of the Mouse Hepatitis Virus A59 Genome.- Identifying Coronavirus Receptors.- Identification of Sugar Residues Involved in the Binding of TGEV to Porcine Brush Border Membranes.- Pseudotyped Vesicular Stomatitis Virus for Analysis of Virus Entry Mediated by SARS Coronavirus Spike Proteins.