Mycobacteria Protocols

Isolation of Mycobacterium Species Genomic DNA.- Isolation of Mycobacterial RNA.- Lipoglycans of Mycobacterium tuberculosis: Isolation, Purification, and Characterization.- Analyzing Lipid Metabolism: Activation and ?-Oxidation of Fatty Acids.- Analysis of Lipid Biosynthesis and Location.- Whole Genome Analysis Using Microarrays.- Use of DNA Microarrays to Study Global Patterns of Gene Expression.- Two-Dimensional Gel Electrophoresis-Based Proteomics of Mycobacteria.- Transport Assays and Permeability in Pathogenic Mycobacteria.- Continuous Culture of Mycobacteria.- Measuring Minimum Inhibitory Concentrations in Mycobacteria.- Rapid Screening of Inhibitors of Mycobacterium tuberculosis Growth Using Tetrazolium Salts.- Electroporation of Mycobacteria.- Ins and Outs of Mycobacterial Plasmids.- The Use of Temperature-Sensitive Plasmids in Mycobacteria.- Heterologous Expression of Genes in Mycobacteria.- Inducible Expression Systems for Mycobacteria.- Assaying Promoter Activity Using LacZ and GFP as Reporters.- Construction of Unmarked Deletion Mutants in Mycobacteria.- Construction of Targeted Mycobacterial Mutants by Homologous Recombination.- Phage Transposon Mutagenesis.- Gene Essentiality Testing in Mycobacterium smegmatis Using Specialized Transduction.- Gene Switching and Essentiality Testing.- Insertion Element IS6110-Based Restriction Fragment Length Polymorphism Genotyping of Mycobacterium tuberculosis.- Typing Mycobacterium tuberculosis Using Variable Number Tandem Repeat Analysis.- Molecular Detection of Drug-Resistant Mycobacterium tuberculosis with a Scanning-Frame Oligonucleotide Microarray.- MycoDB: An Online Database for Comparative Genomics of the Mycobacteria and Related Organisms.- Environmental Amoebae and Mycobacterial Pathogenesis.