2D PAGE: Sample Preparation and Fractionation

Application of Fluorescence Dye Saturation Labeling for Differential Proteome Analysis of 1,000 Microdissected Cells from Pancreatic Ductal Adenocarcinoma Precursor Lesions.- Albumin and Immunoglobulin Depletion of Human Plasma.- Multi-Component Immunoaffinity Subtraction and Reversed-Phase Chromatography of Human Serum.- Immunoaffinity Fractionation of Plasma Proteins by Chicken IgY Antibodies.- Proteomics of Cerebrospinal Fluid: Methods for Sample Processing.- Sample Preparation of Bronchoalveolar Lavage Fluid.- Preparation of Nasal Secretions for Proteome Analysis.- Preparation of Urine Samples for Proteomic Analysis.- Isolation of Cytoplasmatic Proteins from Cultured Cells for Two-Dimensional Gel Electrophoresis.- Sample Preparation of Culture Medium from Madin-Darby Canine Kidney Cells.- Sample Preparation for Mass Spectrometry Analysis of Formalin-Fixed Paraffin-Embedded Tissue.- Metalloproteomics in the Molecular Study of Cell Physiology and Disease.- Protein Extraction from Green Plant Tissue.- The Terminator: A Device for High-Throughput Extraction of Plant Material.- Isolation of Mitochondria from Plant Cell Culture.- Isolation and Preparation of Chloroplasts from Arabidopsis thaliana Plants.- Isolation of Plant Cell Wall Proteins.- Isolation and Fractionation of the Endoplasmic Reticulum from Castor Bean (Ricinus communis) Endosperm for Proteomic Analyses.- Cell Wall Fractionation for Yeast and Fungal Proteomics.- Collection of Proteins Secreted from Yeast Protoplasts in Active Cell Wall Regeneration.- Sample Preparation Procedure for Cellular Fungi.- Isolation and Enrichment of Secreted Proteins from Filamentous Fungi.- Isolation and Solubilization of Cellular Membrane Proteins from Bacteria.- Isolation and Solubilization of Gram-Positive Bacterial Cell Wall-Associated Proteins.- Cell Fractionation of Parasitic Protozoa.