Plant Immunity
Methods and Protocols
Gebonden Engels 2011 2011e druk 9781617379970Samenvatting
A great deal of effort is being invested in understanding the molecular mechanisms through which plants interact with pathogenic microbes. In Plant Immunity: Methods and Protocols, expert researchers in the field describe emerging technologies that can be applied to the most significant outstanding questions faced by scientists studying immunity in plants. The technologies in this detailed volume include methods for examining protein localization, protein complex purification, protein-protein interactions, transient and inducible gene expression, chromatin immunoprecipitation, microaspiration, laser microdissection, purification of fungal haustoria, and genetic manipulation of bacterial and oomycete pathogens. These techniques are applicable to a wide range of topics, including molecular functionality of NB-LRR proteins and other immune signaling components, and functional characterization of effector proteins and other pathogen components that sabotage host immunity. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Specificaties
Lezersrecensies
Inhoudsopgave
Patrick Cournoyer and S. P. Dinesh-Kumar
2. Fragment Complementation and Co-Immunoprecipitation Assays for Understanding R Protein Structure and Function
Peter Moffett
3. Purification of Resistance Protein Complexes Using a Biotinylated Affinity (HPB) Tag
Yiping Qi and Fumiaki Katagiri
4. Biochemical Purification of Native Immune Protein Complexes
James M. Elmore and Gitta Coaker
5. Chromatin Immunoprecipitation (ChIP) to Identify Global Targets of WRKY Transcription Factor Family Members Involved in Plant Immunity
Mario Roccaro and Imre E. Somssich
6. Dose Response to and Systemic Movement of Dexamethasone in the GVG Inducible Transgene System in Arabidopsis
Xueqing Geng and David Mackey
7. Quantifying Alternatively Spliced mRNA via Capillary Electrophoresis
Xue-Cheng Zhang and Walter Gassmann
8. Constructing Haustorium-Specific cDNA Libraries from Rust Fungi
Ann-Maree Catanzariti, Rohit Mago, Jeff Ellis, and Peter Dodds
9. Microaspiration of Esophageal Gland Cells and cDNA Library Construction for Identifying Parasitism Genes of Plant-Parasitic Nematodes
Richard S. Hussey, Guozhong Huang, and Rex Allen
10. Construction of Pseudomonas syringae pv. tomato DC3000 Mutant and Polymutant Strains
Brian H. Kvitko and Alan Collmer
11. A Straightforward Protocol for Electro-Transformation of Phytophthora capsici Zoospores
Edgar Huitema, Matthew Smoker, and Sophien Kamoun
12. Propagation, Storage, and Assays with Hyaloperonospora arabidopsidis, a Model Oomycete Pathogen of Arabidopsis
John M. McDowell, Troy Hoff, Ryan G. Anderson, and Daniel Deegan
13. Assaying Effector Function In planta Using Double-Barreled Particle Bombardment
Shiv D. Kale and Brett M. Tyler
14. Assays for Effector-Mediated Suppression of Programmed Cell Death in Yeast
Yuanchao Wang and Qian Huang
15. Purification of Effector-Target Protein Complexes via Transient Expression in N. benthamiana
Joe Win, Sophien Kamoun, and Alexandra M.E. Jones
16. Imaging Fluorescently-Tagged Phytophthora Effector Proteins Inside Infected Plant Tissue
Petra C. Boevink, Paul R.J. Birch, and Stephen C. Whisson
17. Immunolocalization of Pathogen Effectors
Eric Kemen, Kurt Mendgen, and Ralf T. Voegele
18. Laser Capture Microdissection of Nematode Feeding Cells
Nagabhushana Ithal and Melissa G. Mitchum
19. Laser Microdissection of Plant-Fungus Interaction Sites and Isolation of RNA for Downstream Expression Profiling
Divya Chandran, Noriko Inada, and Mary C. Wildermuth
20. Global Expression Profiling of RNA from Laser Microdissected Cells at Fungal-Plant Interaction Sites
Divya Chandran, Greg Hather, and Mary C. Wildermuth
21. Visualizing Cellular Dynamics in Plant-Microbe Interactions Using Fluorescent-Tagged Proteins
William Underwood, Serry Koh, and Shauna C. Somerville
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