Transgenic Plants

<p>Contents</p><p> </p><p>Preface</p><p> </p><p>Contributors</p><p> </p><p>Part I. Selection AND DETECTION methods</p><p> </p><p>1. Employment of Cytokinin Vectors for Marker-Free and Backbone-Free Transformation </p><p> </p><p>      Craig M. Richael and Caius M. Rommens</p><p> </p><p> </p><p> </p><p>2.         Organophosphorus Hydrolase; a Multifaceted Plant Genetic Marker Which is Selectable, Scorable, and Quantifiable in Whole Seed</p><p> </p><p>T. Scott Pinkerton, James R. Wild, and John A. Howard</p><p> </p><p> </p><p> </p><p>3.         Use of Northern Blotting for Specific Detection of Small RNA Molecules in Transgenic Plants</p><p>Basel Khraiwesh</p><p> </p><p> </p><p> </p><p>Part II. Transformation Technology – Lower Plants</p><p> </p><p>4.         Genetic Transformation of the Model Green Alga Chlamydomonas reinhardtii</p><p> </p><p>Juliane Neupert, Ning Shao, Yinghong Lu, and Ralph Bock</p><p> </p><p> </p><p> </p><p>Part III. Transformation Technology – Rice </p><p> </p><p>5.     A High Efficiency Agrobacterium-mediated Transformation System of Rice (Oryza sativa L.)</p><p> </p><p>Kenjirou Ozawa </p><p> </p><p> </p><p> </p><p>6.         Selection of Transgenic Rice Plants Using a Herbicide Tolerant Form of the Acetolactate Synthase Gene</p><p> </p><p>Masaki Endo, Tsutomu Shimizu and Seiichi Toki</p><p> </p><p> </p><p> </p><p>7.         Visual Selection in Rice—a Strategy for the Efficient Identification of Transgenic Calli Accumulating Transgene Products</p><p> </p><p>Hiroaki Saika, Haruko Onodera and Seiichi Toki</p><p> </p><p> </p><p> </p><p>8.         Characterization of Rice Genes using Heterologous a Full-length cDNA Expression System</p><p> </p><p>Mieko Higuchi, Youichi Kondou, Masaki Mori, Takanari Ichikawa, Minami Matsui</p><p> </p><p> </p><p> </p><p>9. Bioactive Bead-mediated Transformation of Plants with Large DNA Fragments</p><p> </p><p>Naoki Wada, Joyce A. Cartagena, Naruemon Khemkladngoen, and Kiichi Fukui</p><p> </p><p> </p><p> </p><p>Part IV. Transformation Technology – Cereals and Other Monocots </p><p> </p><p> </p><p> </p><p>10.       Agrobacterium-mediated Transformation of Sorghum bicolor Using Immature Embryos</p><p> </p><p>Songul Gurel, Ekrem Gurel, Tamara I. Miller, and Peggy G. Lemaux</p><p> </p><p> </p><p> </p><p>11.       Split-transgene Expression in Wheat</p><p> </p><p>Mario Gils, Myroslava Rubtsova, and Katja Kempe</p><p> </p><p> </p><p> </p><p>12.       Agrobacterium-mediated Transformation of Brachypodium Distachyon</p><p> </p><p>Vera Thole and Philippe Vain</p><p> </p><p> </p><p> </p><p>13.       Transformation of Barley (Hordeum vulgare L.) by Agrobacterium tumefaciens Infection of In Vitro Cultured Ovules </p><p> </p><p>Inger Bæksted Holme, Henrik Brinch-Pedersen, Mette Lange and Preben Bach Holm</p><p> </p><p> </p><p> </p><p>14.       Biolistic Mediated Production of Transgenic Oil Palm</p><p> </p><p>Ghulam Kadir Ahmad Parveez and Bohari Bahariah </p><p> </p><p> </p><p> </p><p>15.       Transformation of Oil Palm using Agrobacterium tumefaciens</p><p> </p><p>Abang Masli Dayang Izawati, Ghulam Kadir Ahmad Parveez, and Mat Yunus Abdul Masani</p><p> </p><p> </p><p> </p><p>Part V. Transformation Technology – Dicots</p><p> </p><p>16.       Highly Efficient Transformation Protocol for Plum (Prunus domestica L.)</p><p>César Petri, Ralph Scorza, and Chinnathambi Srinivasan</p><p> </p><p> </p><p> </p><p>17.       Co-transformation of Grapevine Somatic Embryos to Produce Transgenic Plants Free of Marker Genes</p><p> </p><p>Manjul Dutt, Zhijian T. Li, Sandanand A. Dhekney and Dennis J. Gray</p><p> </p><p> </p><p> </p><p>18.       Initiation and Transformation of Grapevine Embryogenic Cultures</p><p> </p><p>Sandanand A. Dhekney, Zhijian T. Li, Manjul Dutt, and Dennis J. Gray</p><p> </p><p> </p><p> </p><p>19.       Development of Highly Efficient Genetic Transformation Protocols for Table Grape Sugraone and Crimson Seedless</p><p> </p><p>Mercedes Dabauza and Leonardo Velasco</p><p> </p><p> </p><p> </p><p>20.       Cotton Pistil Drip Transformation Method</p><p> </p><p>Tianzhen Zhang and Tianzhi Chen </p><p> </p><p> </p><p> </p><p>21.       Enhanced Agrobacterium-mediated Transformation of Embryogenic Calli of Upland Cotton</p><p> </p><p>Tian-zhen Zhang and Shen-jie Wu </p><p> </p><p> </p><p> </p><p>22.       Targeted Biolistics for Improved Transformation of Impatiens balsamina</p><p> </p><p>Andy Wetten, Jean-Luc Thomas, Alina Wagiran, Tinashe Chiurugwi</p><p> </p><p> </p><p> </p><p>23.       A Protocol for Transformation of Torenia</p><p> </p><p>Ryutaro Aida</p><p> </p><p> </p><p> </p><p>24.       Efficient Modification of Floral Traits by Heavy-Ion Beam Irradiation on Transgenic Torenia</p><p> </p><p>Norihiro Ohtsubo, Katsutomo Sasaki, Ryutaro Aida, Hiromichi Ryuto, Hiroyuki Ichida, Yoriko Hayashi, and Tomoko Abe</p><p> </p><p> </p><p> </p><p>Part VI. Gene Targeting, Silencing and Directed Mutation</p><p> </p><p>25.       </p><p> </p><p>Isam Fattash, Basel Khraiwesh , M. Asif Arif and Wolfgang Frank</p><p> </p><p>26.     High Frequency of Single-copy T-DNA Transformants Produced after Floral Dip in CRE-expressing Arabidopsis Plants</p><p> </p><p>Annelies De Paepe, Sylvie De Buck, Jonah Nolf, and Ann Depicker</p><p> </p><p> </p><p> </p><p>27.       A Developmentally Regulated Cre-lox system to Generate Marker-free Transgenic Brassica napus Plants</p><p> </p><p>Lilya Kopertekh, Inge Broer, and Joachim Schiemann </p><p> </p><p> </p><p> </p><p>28.       Exploiting Multisite Gateway and pENFRUIT Plasmid Collection for Fruit Genetic EngineeringLeandro H. Estornell, Antonio Granell, and Diego Orzaez</p><p> </p><p> </p><p>29.       A One-time Inducible Transposon to Create Knockout Mutants in Rice </p><p> </p><p>Yuh-Chyang Charng</p><p> </p><p> </p><p> </p><p>30.       Marker-free Gene Targeting by Recombinase-mediated Cassette Exchange (RMCE)</p><p> </p><p>Hiroyasu Ebinuma, Kazuya Nanto, Saori Kasahara, and Atsushi Komamine</p><p> </p><p> </p><p> </p><p>31.       Targeting DNA to a Previously Integrated Transgenic Locus Using Zinc Finger Nucleases</p><p> </p><p>Tonya L. Strange Moynahan and Joseph F. Petolino</p><p> </p><p> </p><p> </p><p>32.       Double-strand Break-induced Targeted Mutagenesis in Plants</p><p> </p><p>L. Alexander Lyznik, Vesna Djukanovic, Meizhu Yang, and Spencer Jones</p><p> </p><p> </p><p> </p><p> </p><p> </p><p>Part VII. Metabolic EnginEering and Pharming</p><p> </p><p>33.       Combinatorial Genetic Transformation of Cereals and the Creation of Metabolic Libraries for the Carotenoid Pathway</p><p> </p><p>Gemma Farre, Shaista Naqvi, Georgina Sanahuja, Chao Bai, Uxue Zorrilla-López, Sol M. Rivera, Ramon Canela, Gerhard Sandman, Richard M. Twyman, Teresa Capell, Changfu Zhu, and Paul Christou</p><p> </p><p> </p><p> </p><p>34.       Production of a His-Tagged Canecystatin in Transgenic Sugarcane</p><p> </p><p>Flavio Henrique-Silva and Andrea Soares-Costa</p><p> </p><p> </p><p> </p><p>35.       Plastid Transformation as an Expression Tool for Plant-Derived Biopharmaceuticals</p><p> </p><p>Nunzia Scotti and Teodoro Cardi</p><p> </p><p> </p><p> </p><p>36.       Use of a Callus-Specific Selection System to Development Transgenic Rice Seed Accumulating a High Level of Recombinant Protein </p><p> </p><p>Yuhya Wakasa and Fumio Takaiwa</p><p> </p><p> </p><p> </p><p>Part VIII. Field TESTS</p><p> </p><p>37.       How to Grow Transgenic Arabidopsis in the Field</p><p> </p><p>Hanna Johansson Jänkänpää and Stefan Jansson</p>