DNA Repair Protocols

DNA repair: Front and Center and Not Going Away!.-Identification of Genes Required for Damage Survival using a Cell Based RNAi Screen against the Drosophila Genome.-Isolating Genes Involved with Genotoxic Drug Response in the Nematode Caenorhabditis elegans using Genome-wide RNAi Screening.-Establishment of the DNA Repair-defective Mutants in DT40 Cells.-Electrophoretic Mobility Shift Assays for Protein-DNA Complexes Involved in DNA Repair.-The Comet Assay A Sensitive Genotoxicity Test for the Detection of DNA Damage and Repair.-Detection of DNA Damage by Comet Fluorescence in situ Hybridization (Comet-FISH).-Quantitative DNA Damage and Repair Measurement with the Yeast Comet Assay.-Analysis of DNA Damage and Repair in Nuclear and Mitochondrial DNA of Animal Cells using Quantitative PCR.-In vitro DNA Mismatch Repair in Human Cells.-Eukaryotic DNA Mismatch Repair in vitro.-Immuno-slot Blot Assay for Detection of UVR-mediated DNA Damage.-Quantification of DNA Photoproductions in Mammalian Cell DNAUsing Radioimmunoassay.-Measuring the Formation and Repair of UV Damage at the DNA Sequence Level by Ligation-mediated PCR.-Construction of Plasmids Containing Site-specific DNA Interstrand Crosslinks for Biochemical and Cell Biological Studies.-Replication-coupled DNA Interstrand Crosslink Repair in  Xenopus Egg Extracts.-In Vitro Base Excision Repair using Mammalian Cell Extracts.-Using Arabidopsis Cell Extracts to Monitor Repair of DNA Base Damage in vitro.-In vitro monitoring of Base Excision Repair in Saccharomyces cerevisiae.-Mitochondrial Base Excision Repair Assays.-O-Hydroxylamine-Coupled Alkaline Gel Electrophoresis Assay for the Detection and Measurement of DNA Single-Strand Breaks.-DNA DoubleStand Break Damage and Repair Assessed by Pulse-field Gel Electrophoresis.-Quantitative Amplification of Single Stranded DNA (QAOS).-Simple, Non-radioactive Measurement of Single-stranded DNA at Telomeric, Sub-telomeric and Genomic Loci in Budding Yeast.-Monitoring DNA Recombination Initiated by HO Endonuclease.-Monitoring I-SceI Induced Double Strand Break Repair in DT40 Cells.-I-SceI-based Assays to Examine Distinct Repair Outcomes of Mammalian Chromosomal Double Strand Breaks.-Studying Repair of a Single Protein-bound Nick in vivo using the Flp-nick System.-Measurement of Diversification in the Immunoglobulin Light Chain Gene of DT40 Cells.-Live cell microscopy of DNA damage response in Saccharomyces cerevisiae.-Use of Gene Targeting to Study Recombination in Mammalian Cell DNA

Repair Mutants.-In vitro Rejoining of Double Strand Breaks in Genomic DNA.-An In Vitro DNA Double-Strand Break Repair Assay Based on End Joining of Defined Duplex Oligonucleotides.-Assays of Bypass Replication of Genotoxic Lesions in Cell-Free Extracts

Nana Nikolaishvili-Feinberg and Marila Cordeiro-Stone.-Quantitative Measurement of Translesion DNA Synthesis in Mammalian Cells.-Detection of PCNA Modifications in Saccharomyces cerevisiae.-In vitro PCNA Modification Assays.-Analysis of Inhibition of DNA Replication in Irradiated Cells using the SV40 Based in vitro assay of DNA Replication.-CHK1 Kinase Activity Assay.-Histone H2AX Phosphorylation: A Marker for DNA Damage.

Repair Mutants.-In vitro Rejoining of Double Strand Breaks in Genomic DNA.-An In Vitro DNA Double-Strand Break Repair Assay Based on End Joining of Defined Duplex Oligonucleotides.-Assays of Bypass Replication of Genotoxic Lesions in Cell-Free Extracts

Nana Nikolaishvili-Feinberg andMarila Cordeiro-Stone.-Quantitative Measurement of Translesion DNA Synthesis in Mammalian Cells.-Detection of PCNA Modifications in Saccharomyces cerevisiae.-In vitro PCNA Modification Assays.-Analysis of Inhibition of DNA Replication in Irradiated Cells using the SV40 Based in vitro assay of DNA Replication.-CHK1 Kinase Activity Assay.-Histone H2AX Phosphorylation: A Marker for DNA Damage.

Repair Mutants.-In vitro Rejoining of Double Strand Breaks in Genomic DNA.-An In Vitro DNA Double-Strand Break Repair Assay Based on End Joining of Defined Duplex Oligonucleotides.-Assays of Bypass Replication of Genotoxic Lesions in Cell-Free Extracts

Nana Nikolaishvili-Feinberg and Marila Cordeiro-Stone.-Quantitative Measurement of Translesion DNA Synthesis in Mammalian Cells.-Detection of PCNA Modifications in Saccharomyces cerevisiae.-In vitro PCNA Modification Assays.-Analysis of Inhibition of DNA Replication in Irradiated Cells using the SV40 Based in vitro assay of DNA Replication.-CHK1 Kinase Activity Assay.-Histone H2AX Phosphorylation: A Marker for DNA Damage.