Plant Chemical Genomics
Methods and Protocols
Gebonden Engels 2013 2014e druk 9781627035910Samenvatting
Plant Chemical Genomics: Methods and Protocols , expert researchers in the field detail many of the techniques used for identification of bioactive compounds from a large selection of fields in plant biology including plant pathogenesis, immune responses, small RNA processing, endomembrane trafficking, lipids, plant hormone signaling and cell wall. The presentation of these and other examples as well as synthetic chemistry, computation and target identification provides a comprehensive overview of the practical aspects of chemical biology that are possible in plant systems. Small molecules and natural products that produce a phenotype in plants have been known for decades. The agrichemical industry has utilized small molecules based on known natural molecules such as auxins, cytokinins, abscissic acid and other growth regulators. In recent years, the screening and characterization of novel small molecules has enhanced our understanding of protein function, metabolic, signaling and endomembrane pathways, and their interactions in basic plant research.
Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Specificaties
Lezersrecensies
Inhoudsopgave
1. Fully-automated Compound Screening in Arabidopsis thaliana Seedlings
Dominique Audenaert, Long Nguyen, Bert De Rybel, and Tom Beeckman
2. Time-profiling Fluorescent Reporters in the Arabidopsis Root
Antoine P. Larrieu, Andrew P. French, Tony P. Pridmore, Malcolm J. Bennett and Darren M. Wells
3. Screening for Bioactive Small Molecules by in vivo Monitoring of Luciferase-based Reporter Gene Expression in Arabidopsis thaliana
Christian Meesters and Erich Kombrink
Section II: Chemical Screening
4. Application of Yeast-two Hybrid Assay to Chemical Genomic Screens: A High-throughput System to Identify Novel Molecules Modulating Plant Hormone Receptor Complexes
Andrea Chini
5. High-throughput Screening of Small Molecule Libraries for Inducers of Plant Defense Responses
Colleen Knoth and Thomas Eulgem
6. Using a Reverse Genetics Approach to Investigate Small Molecule Activity
Siamsa M. Doyle and Stéphanie Robert
7. Investigating the Phytohormone Ethylene Response Pathway by Chemical Genetics
Lee-Chung, Lin, Chiao-Mei Chueh, and Long-Chi Wang
8. Screening for Inhibitors of Chloroplast Galactolipid Synthesis Acting in membrano and in Planta
Laurence Boudière and Eric Maréchal
9. Forward Chemical Screening of Small RNA Pathways
Yifan Lii and Hailing Jin
10. Identification and Use of Fluorescent Dyes for Plant Cell Wall Imaging Using High-Throughput Screening
Charles T. Anderson and Andrew Carroll
11. High-throughput Identification of Chemical Endomembrane Cycling Disruptors Utilizing Tobacco Pollen
Michelle Q. Brown, Nolan Ung, Natasha V. Raikhel, and Glenn R. Hicks
12. Plant Chemical Genomics: Gravity Sensing and Response
Marci Surpin 13. Screening Chemical Libraries for Compounds that Affect Protein Sorting to the Yeast Vacuole
Jan Zouhar
Section III: Cheminformatics
14. The use of Multi-drug Approach to Uncover New Players of the Endomembrane System Trafficking Machinery
Urbina D, Pérez-Henríquez P, and Norambuena L 15. Cheminformatic Analysis of High-Throughput Compound Screens
Tyler W. H. Backman and Thomas Girke
16. Endomembrane Dissection Using Chemically-Induced Bioactive Clusters
Natasha Worden, Thomas Girke, and Georgia Drakakaki 17. Statistical Molecular Design: A Tool to Follow Up Hits From Small Molecule ScreeningAnders E. G. Lindgren, Andreas Larsson, Anna Linusson, and Mikael Elofsson
Section IV: Target Identification
18. Early Stage Hit Triage for Plant Chemical Genetic Screens and Target Site Identification
Terence A. Walsh
19. Screening for Gene Function using the FOX (Full-length cDNA OvereXpressor gene) Hunting System
Mieko Higuchi, Minami Matsui
Section V: Hormone Transport and Metabolite Profiling
20. Quantification of Stable Isotope Label in Metabolites via Mass Spectrometry
Jan Huege, Jan Goetze, Frederik Dethloff, Bjoern Junker, and Joachim Kopka
21. 1H NMR Based Metabolomics Methods for Chemical Genomics Experiments
Daniel J. Orr, Gregory A. Barding, Jr., Christiana E. Merrywell, Glenn R. Hicks, Natasha V. Raikhel, and Cynthia K. Larive
22. Determination of Auxin Transport Parameters on the Cellular Level
Jan Petrášek, Martina Laňková, and Eva Zažímalová
23. Analyzing the in vivo Status of Exogenously Applied Auxins: A HPLC-based Method to Characterize the Intracellularly Localized Auxin Transporters
Sibu Simon, Petr Skůpa, Petre I. Dobrev, Jan Petrášek, Eva Zažímalová, and Jiří Friml
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