Sirtuins

Methods and Protocols

Gebonden Engels 2013 2013e druk 9781627036368
€ 183,99
Levertijd ongeveer 9 werkdagen
Gratis verzonden

Samenvatting

Featuring a diverse array of model organisms and scientific techniques, Sirtuins: Methods and Protocols collects detailed contributions from experts in the field addressing this vital family of genes. Opening with methods to generate sirtuin biology tools, the book continues by covering methods to identify sirtuin substrates, to measure sirtuin activity, and to study sirtuin biology. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 Comprehensive and easy to use, Sirtuins: Methods and Protocols presents detailed protocols for sirtuin research that can be followed directly or modified to investigate new areas of sirtuin biology.

Specificaties

ISBN13:9781627036368
Taal:Engels
Bindwijze:gebonden
Aantal pagina's:314
Uitgever:Humana Press
Druk:2013

Lezersrecensies

Wees de eerste die een lezersrecensie schrijft!

Inhoudsopgave

<p>Introduction: Sirtuins in Aging and Diseases.- Sirtuins in Yeast: Phenotypes and Tools.- Studying the C. elegans Sirtuins.- Genetic and Biochemical Tools for Investigating Sirtuin Function in Drosophila melanogaster.- Generating Mammalian Sirtuin Tools for Protein-Interaction Analysis.- Mass Spectrometry-Based Detection of Protein Acetylation.- SILAC-Based Quantification of Sirt1-Responsive Lysine Acetylome.- Targeted Quantitation of Acetylated Lysine Peptides by Selected Reaction Monitoring Mass Spectrometry.- Identification of Deacetylase Substrates with the Biotin Switch Approach.- Assaying Chromatin Sirtuins.- Measurement of Sirtuin Enzyme Activity Using a Substrate-Agnostic Fluorometric Nicotinamide Assay.- Detecting Sirtuin-Catalyzed Deacylation Reactions Using <sup>32</sup>P-Labeled NAD and Thin-Layer Chromatography.- Chemical Acetylation And Deacetylation.- Accurate Measurement of Nicotinamide Adenine Dinucleotide (NAD<sup>+</sup>) with High-Performance Liquid Chromatography.- In Vivo Measurement of the Acetylation State of Sirtuin Substrates as a Proxy for Sirtuin Activity.- Oxygen Flux Analysis to Understand the Biological Function of Sirtuins.- The Emerging Links Between Sirtuins and Autophagy.- Methods to Study the Role of Sirtuins in Genome Stability.- Circadian Measurements of Sirtuin Biology.- Utilizing Calorie Restriction to Evaluate the Role of Sirtuins in Healthspan and Lifespan of Mice.</p>

Managementboek Top 100

€ 183,99
Levertijd ongeveer 9 werkdagen
Gratis verzonden

Rubrieken

    Personen

      Trefwoorden

        Sirtuins