Basic Cell Culture Protocols

Culture of Primary Adherent Cells and a Continuously Growing Nonadherent Cell Line.- Detection of Mycoplasma Contaminations.- Eradication of Mycoplasma Contaminations.- Authentication of Scientific Human Cell Lines.- Cytogenetic Analysis of Cell Lines.- Human and Mouse Hematopoietic Colony-Forming Cell Assays.- Isolation and Culture of Murine Macrophages.- Isolation and Culture of Human Macrophages.- Development of T-Lymphocytes in Mouse Fetal Thymus Organ Culture.- In Vitro Generation of Lymphocytes From Embryonic Stem Cells.- Hematopoietic Development of Human Embryonic Stem Cells in Culture.- Generation of Murine Stromal Cell Lines.- Culture of Human and Mouse Mesenchymal Cells.- Isolation, Purification, and Cultivation of Murine and Human Keratinocytes.- Isolation and Culture of Primary Human Hepatocytes.- Primary Kidney Proximal Tubule Cells.- Enzymatic Dissociation and Culture of Normal Human Mammary Tissue to Detect Progenitor Activity.- Generation and Differentiation of Neurospheres From Murine Embryonic Day 14 Central Nervous System Tissue.- Isolation and Culture of Skeletal Muscle Myofibers as a Means to Analyze Satellite Cells.- Adult Ventricular Cardiomyocytes.- Isolation and Culture of Primary Endothelial Cells.- Studying Leukocyte Rolling and Adhesion In Vitro Under Flow Conditions.- Isolation and Characterization of Side Population Cells.- Scalable Production of Embryonic Stem Cell-Derived Cells.