Gene Therapy Protocols

Purification of Retrovirus Particles Using Heparin Affinity Chromatography.- Scaleable Purification of Adenovirus Vectors.- Quantifying the Titer and Quality of Adenovirus Stocks.- Chromatography-Based Purification of Adeno-Associated Virus.- Spectroscopic Methods for the Physical Characterization and Formulation of Nonviral Gene Delivery Systems.- Real-Time Multiple Particle Tracking of Gene Nanocarriers in Complex Biological Environments.- Production of Lentiviruses Displaying “Early-Acting” Cytokines for Selective Gene Transfer into Hematopoietic Stem Cells.- Fiber-modified Adenoviruses for Targeted Gene Therapy.- PEGylated Adenovirus for Targeted Gene Therapy.- Transposon-Based Mutagenesis Generates Diverse Adeno-Associated Viral Libraries with Novel Gene Delivery Properties.- Photochemical Enhancement of DNA Delivery by EGF Receptor Targeted Polyplexes.- Reducing the Genotoxic Potential of Retroviral Vectors.- Evaluation of Promoters for Use in Tissue-Specific Gene Delivery.- Adenovirus-Mediated Transduction of Auto- and Dual-Regulated Transgene Expression in Mammalian Cells.- Regulated Expression of Adenoviral Vectors-Based Gene Therapies.- Liver-Directed Gene Therapy Using the Sleeping Beauty Transposon System.- Generation and Functional Analysis of Zinc Finger Nucleases.- Conditional Gene Expression and Knockdown Using Lentivirus Vectors Encoding shRNA.- Nanoparticle-Mediated Gene Delivery to the Lung.- Retroviral-Mediated Gene Therapy for the Differentiation of Primary Cells into a Mineralizing Osteoblastic Phenotype.- In Vivo siRNA Delivery to the Mouse Hypothalamus Shows a Role of the Co-Chaperone XAP2 in Regulating TRH Transcription.- Efficient Retroviral Gene Transfer to Epidermal Stem Cells.