Plant-Pathogen Interactions

<p> Part 1:  Bioinformatics</p><p> 1.             Galaxy as a Platform for Identifying Candidate Pathogen Effectors<br>Peter J. A. Cock and Leighton Pritchard</p><p>2.             Bioinformatic analysis of expression data to identify effector candidates<br>Adam J. Reid and  John T. Jones </p><p>3.             Two-dimensional data binning for the analysis of genome architecture in filamentous plant pathogens and other eukaryotes<br>Diane G.O. Saunders, Joe Win, Sophien Kamoun, and  Sylvain Raffaele</p><p>4.             On The Statistics Of Identifying Candidate Pathogen Effectors<br>Leighton Pritchard amd David Broadhurst</p><p> </p><p>Part 2:  Cell Biology</p><p> 5.      High-throughput imaging of plant immune responses<br>Martina Beck, Ji Zhou, Christine Faulkner, and Silke Robatzek</p><p>6.      In vivo protein-protein interaction studies with BiFC: conditions, cautions and caveats<br>Petra Boevink, Hazel McLellan, Tatyana Bukharova, Stefan Engelhardt, and  Paul R.J. Birch</p><p>7.      Particle bombardment-mediated transient expression to identify localization signals in plant disease resistance proteins and target sites for the proteolytic activity of pathogen effectors<br>Daigo Takemoto and David Jones</p><p>8.      Purification of fungal haustoria from infected plant tissue by flow cytometry<br>Diana P. Garnica and John P. Rathjen</p><p> </p><p>Part 3:  From assays of pathogen virulence to effector function</p><p> 9.      Functional Characterisation of Nematode Effectors in Plants<br>Axel A. Elling and John T. Jones</p><p>10.  Silencing of aphid genes by feeding on stable transgenic Arabidopsis thaliana<br>Alexander D. Coleman, Marco Pitino, and Saskia A. Hogenhout </p><p>11.  Leaf-disc assay based on transient over-expression in Nicotiana benthamiana to allow functional screening of candidate effectors from aphids<br>Patricia A. Rodriguez, Saskia A. Hogenhout, and Jorunn I.B. Bos</p><p>12.  A growth quantification assay for Hyaloperonospora arabidopsidis isolates in Arabidopsis thaliana<br>Daniel F.A. Tomé, Jens Steinbrenner, and Jim L. Beynon</p><p>13.  Simple quantification of in planta fungal biomass<br>Michael Ayliffe, Sambasivam K Periyannan, Angela Feechan, Ian Dry, Ulrike Schumann, Evans Lagudah, and Anthony Pryor</p><p>14. Virus-induced Gene Silencing and Agrobacterium Tumefaciens-Mediated Transient Expression in Nicotiana Tabacum</p><p>Zhao Zhang and Bart P.H.J. Thomma</p><p>15.  DIGE-ABPP by click chemistry: Pairwise comparison of serine hydrolase activities from the apoplast of infected plants</p><p>Tram Ngoc Hong and Renier A.L. van der Hoorn</p><p>16. A Simple and Fast Protocol for Protein Complex Immunoprecipitation (Co-IP) of Effector-Host Protein Complexes</p><p>Jens Steinbrenner, Matthew Eldridge, Daniel F.A. Tomé, and Jim L. Beynon</p><p>17.  An Arabidopsis and Tomato Mesophyll Protoplast System for fast Identification of early MAMP-triggered Immunity-Suppressing Effectors<br>Malou Fraiture, Xiangzi Zheng, and  Frédéric Brunner</p><p>18.  Production of RXLR effector proteins for structural analysis by X-ray crystallography<br>Richard K. Hughes and Mark J. Banfield</p><p> </p><p>Part 4:  Methods to identify Resistance genes and avirulence genes</p><p> 19.  The Do’s and Don’ts of effectoromics<br>Juan Du and Vivianne G.A.A. Vleeshouwers</p><p>20.  Protoplast cell death assay to study Magnaporthe oryzae AVR gene function in rice<br>Hiroyuki Kanzaki, Kentaro Yoshida, Hiromasa Saitoh, Muluneh Tamiru, and Ryohei Terauchi</p><p>21.  A Bacterial Type III Secretion Based Delivery System for Functional Assays of Fungal Effectors in Cereals<br>Narayana M. Upadhyaya, Jeffery G. Ellis, and Peter N. Dodds</p><p>22.  Capture arrays to annotate resistance genes in plant genomes and to accelerate plant resistance gene discovery<br>Florian Jupe, Xinwei Chen, Walter Verweij, Kamil Witek, Jonathan D.G. Jones,  and Ingo Hein</p><p>