Advanced Technologies for Protein Complex Production and Characterization

<p>Preface. Complex Biologics: Engineering the Tools and the Hosts. M. Cristina Vega, Francisco J. Fernández and Imre Berger</p>

<p>Section 1: Introduction</p>

<p>1. Protein complex production from the drug discovery standpoint;&nbsp;Ismail Moarefi&nbsp; &nbsp; &nbsp;</p>

<p><2. Choose a suitable expression host: a survey of available protein production platforms;&nbsp;Francisco J. Fernández and M. Cristina Vega</p>

<p>Section 2: Prokaryotic expression hosts</p>

<p>3. ACEMBL Tool-Kits for High-Throughput Multigene Delivery and Expression in Prokaryotic and Eukaryotic Hosts;&nbsp;Yan Nie, Maxime Chaillet, Christian Becke, Matthias Haffke, Martin Pelosse, Daniel Fitzgerald, Ian Collinson, Christiane Schaffitzel and Imre Berger</p>

<p>4. Complex reconstitution and characterization by combining co-expression techniques in Escherichia coli with high-throughput;&nbsp;Renaud Vincentelli and Christoph Romier</p>

<p>5. Membrane protein production in E. coli for applications in drug discovery;&nbsp;Harm Jan (Arjan) Snijder and Jonna Hakulinen</p><p>6. Cell-free Synthesis of Macromolecular Complexes;&nbsp;Matthieu Botte, Aurélien Deniaud and Christiane Schaffitzel&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;</p>

<p>7. A Bacillus megaterium system for the production of recombinant proteins and protein complexes;&nbsp;Rebekka Biedendieck</p>

<p>8. Protein complex production in alternative prokaryotic hosts;&nbsp;Sara Gómez, Miguel López-Estepa, Francisco J. Fernández and M. Cristina Vega&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;&nbsp;</p>

<p>Section 3: Lower Eukaryotic expression hosts</p>

<p>9. Production of Protein Complexes in Non-methylotrophic and Methylotrophic Yeasts;&nbsp;Francisco J. Fernandez, Miguel López-Estepa, Javier Querol-García and M. Cristina Vega</p>

<p>10. Leishmania tarentolae for the Production of Multi-Subunit Complexes;&nbsp;Tomoaki Niimi</p>

<p>11. Alternative Eukaryotic Expression Systems for the Production of Proteins and Protein Complexes;&nbsp;Sara Gómez, Miguel López-Estepa, Francisco J. Fernández, Teresa Suárez and M. Cristina Vega</p>

<p>Section 4: Higher Eukaryotic expression hosts</p>

<p>12. Fundamentals of baculovirus expression and applications;&nbsp;Tom A. Kost and Christopher W. Kemp</p>

<p>13. The MultiBac Baculovirus / Insect Cell Expression Vector System for Producing Complex Protein Biologics;&nbsp;Duygu Sari, Kapil Gupta, Deepak Balaji Govinda Raj, Alice Aubert, Petra Dmcova, Frederic Garzoni, Daniel Fitzgerald and Imre Berger&nbsp;&nbsp;</p>

<p>14. Fundamentals of expression in mammalian cells;&nbsp;Michael R. Dyson&nbsp;</p>

15. Assembling multi-subunit complexes using mammalian expression;&nbsp;Bahar Baser and Joop van den Heuvel</p>

<p>Section 5: Plant expression</p>

<p>16. Microalgae as solar-powered protein factories;&nbsp;Franziska Hempel and Uwe G. Maier</p>

<p>17. Strategies and methodologies for the co-expression of multiple proteins in plants;&nbsp;Albert Ferrer, Montserrat Arró, David Manzano and Teresa Altabella&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;&nbsp;</p>

<p>18. Transient expression systems in plants—potentialities and constraints;&nbsp;Tomas Canto&nbsp; &nbsp; &nbsp; &nbsp; &nbsp;</p>

Section 6: Complex reconstitution</p>

<p>19. Complex reconstitution from individual protein modules;&nbsp;Jérome Basquin, Michael Taschner and Esben Lorentzen&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;</p>

<p>20. Structural reconstruction of protein-protein complexes involved in intracellular signaling;&nbsp;Klára Kirsch, Péter Sok, and Attila Remény&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;</p>

<p>Section 7: Biophysical methods to assess protein complexes</p>

<p>21. The use of small-angle scattering for the characterization of multi subunit complexes;&nbsp;Adam Round</p><p>22. Application of nuclear magnetic resonance and hybrid methods to structure determination of complex systems. Filippo Prischi and Annalisa Pastore</p>