Methods in Endothelial Cell Biology

I: Isolation and Culture of Endothelial Cells.- 1 Isolation, Purification, and Culture of Human Micro- and Macrovascular Endothelial Cells.- 2 Isolation of Mouse Cardiac Microvascular Endothelial Cells.- 3 Isolation, Culture, and Differentiation of Circulating Endothelial Precursor Cells.- 4 Embryonic Stem Cell-Derived Endothelial Cells.- 5 Detection, Isolation, and Culture Of Lymphatic Endothelial Cells.- 6 Three-Dimensional Spheroid Culture of Endothelial Cells.- 7 Immortalization of Endothelial Cells.- 8 Viral Gene Transfer Into Endothelial Cells.- II: Functional Assays in Vitro.- 9 Leukocyte Adhesion to Endothelium in Vitro Under Shear Stress.- 10 Application of Biomechanical Forces on Endothelial Cells.- 11 Endothelial Cell Permeability Assays in Culture.- 12 Three-Dimensional in Vitro Angiogenesis Assays.- 13 Rat Aortic Ring Assay of Angiogenesis.- 14 Endothelial Cell Chemotaxis and Chemokinesis Assays.- 15 FACS Analysis of Endothelial Cells.- 16 Application of the RNA Interference (RNAi) Technology to Angiogenesis Research.- III: Functional Assays in Vivo.- 17 Murine Bone Marrow Transplantation Models that Enable the Study of EPC Recruitment.- 18 Balloon Denudation of Blood Vessels.- 19 Peripheral Hind Limb Ischemia Models.- 20 In Vivo Matrigel Angiogenesis Assays.- 21 Analysis of Vascular Permeability in vivo.- 22 Validating the Manipulation of Endothelial Cell signaling in Vivo.- 23 Chick Chorioallantoic Membrane Assay: Growth Factor and Tumor-induced Angiogenesis and Lymphangiogenesis.- 24 Corneal Angiogenesis Assay.- IV: Detection of Endothelial Cells.- 25 Basic Blood Vessel Immunohistochemistry.- 26 Double-Staining Immunohistochemistry of Blood Vessels.- 27 In Situ Hybridization Analysis of Vascular Endothelium.- 28 High-Resolution in Situ Confocal Analysisof Endothelial Cells.- 29 Whole Mount Analysis of the Embryonic Vasculature.- 30 Methods for Visualizing Intact Blood Vessels.- 31 Intravital Multi-Fluorescence Microscopy.- 32 Corrosion Cast Analysis of Blood Vessels.- Suppliers.- Endothelial Cell Biology on the Internet.