Flow Cytometry and Cell Sorting

I Flow Cytometry.- 1 Operation of a Flow Cytometer.- II Immunofluorescence.- 2 Conjugation of Fluorochromes, Haptens, and Phycobiliproteins to Antibodies.- 3 Immunofluorescence: Basic Considerations.- 4 Multicolor Immunofluorescence Analysis.- 5 Combined Intracellular and Surface Staining: Immunofluorescence of Cytokines in T Cells.- 6 Cytometry and Sorting of Live Cells According to Their Secreted Products.- 7 Cytometry of Rare Surface Molecules by Magnetofluorescent Liposomes.- 8 Scatchard Analysis by Flow Cytometry.- 9 Microbead Assay for Quantification of Neuronal Adhesion Molecule Interaction by Flow Cytometry.- III DNA and Proliferation.- 10 Preparation and Staining of Fixed Cells for High-Resolution DNA Analysis.- 11 Simultaneous Flow Cytometric Detection of Bromodeoxyuridine Incorporation and Cell Surface Marker Expression.- 12 High-Resolution Cell Cycle Analysis: The Flow Cytometric Bromodeoxyuridine-Hoechst Quenching Technique.- IV Cellular Activation and Biochemistry.- 13 Cell Activation: Indo-1 Ratiometric Analysis of Intracellular Ionized Calcium.- 14 Detection and Purification of Rare Responders by Fixed-Time Flow Cytometry.- 15 Biochemical Parameters of Cells Function.- 16 The FACS-Gal Assay.- 17 Ligand Acidification by Nonadherent Cells.- V Cell Sorting.- 18 Powerful Preselection.- 19 Magnetic Selection of Transfected Cells.- 20 High Gradient Magnetic Cells Sorting.- 21 Setup of a Flow Sorter.- 22 Sorting of Rare Cells.- 23 Fluorescence-Activated Chromosome Sorting.- 24 Analysis and Sorting of Plant Chromosomes by Flow Cytometry.- 25 Large Particle Sorting.- VI Safety.- 26 Biological and Laser Safety.- Appendices: Birgit Mechtold and Andreas Radbruch.- Suppliers.- Cytometry on the Internet.