R.C. Bird,
Bruce F. Smith
Genetic Library Construction and Screening
Advanced Techniques and Applications
Paperback Engels 2012 2002e druk 9783642477331Levertijd ± 9 werkdagen
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Samenvatting
Designed as an introductory text the authors cover all core strategies in the application of modern recombinant DNA technology. The first chapters directly address the applications of polymerase chain reaction to a variety of problems in DNA cloning that are, or have been, extremely challenging using more traditional approaches and technologies. These include cDNA cloning and transcript mapping, mutagenesis as well as the cloning of very long transcripts and protocols using limiting amounts of total RNA. Further chapters describe approaches to subtractive cloning technologies as well as novel specialized expression cloning and library screening strategies. The handbook contains detailed step-by-step protocols and extensive hands-on advice.
Specificaties
ISBN13:9783642477331
Taal:Engels
Bindwijze:Paperback
Aantal pagina's:241
Uitgever:Springer Berlin Heidelberg
Druk:2002
Serie:Springer Lab Manuals
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